Regeneration of comparative genomic hybridization oligonucleotide microarrays with dimethylurea

被引:6
作者
Ronowicz, Anna [1 ]
Brzeskwiniewicz, Magdalena [2 ]
Madanecki, Piotr [1 ]
Buckley, Patrick G. [3 ]
Orlowska, Ewa [1 ]
Ochocka, J. Renata [1 ]
Limon, Janusz [2 ]
Piotrowski, Arkadiusz [1 ]
机构
[1] Med Univ Gdansk, Dept Biol & Pharmaceut Bot, PL-80416 Gdansk, Poland
[2] Med Univ Gdansk, Dept Biol & Genet, PL-80210 Gdansk, Poland
[3] Beaumont Hosp, Dept Neuropathol, Dublin 9, Ireland
关键词
Reuse; Regeneration; Oligonucleotide; Microarray; Comparative genomic hybridization; Fluorescent labeling; Dimethylurea; DNA;
D O I
10.1016/j.ab.2012.04.013
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Reuse of materials in DNA hybridization-based methods has been known since the advent of Southern membranes. Array-based comparative genomic hybridization is essentially Southern hybridization with multiple probes immobilized on a solid surface. We show that comparative genomic hybridization microarrays fabricated with maskless array synthesizer technology can be used up to four times with the application of 1,3-dimethylurea as an array-stripping agent. We reproducibly detected chromosomal aberrations (0.6-22.4 Mb in size) in four hybridization rounds using regenerated microarray slides. We also demonstrated that regenerated arrays can detect smaller alterations (16-200 kbp), such as common copy number variants, as well as complex aberration profiles in tumor DNA. (C) 2012 Elsevier Inc. All rights reserved.
引用
收藏
页码:91 / 93
页数:3
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