Platycodon grandiflorum modifies adipokines and the glucose uptake in high-fat diet in mice and L6 muscle cells

被引:29
作者
Ahn, Young-Min [1 ]
Kim, Su Kang [2 ]
Kang, Ji-Suck [1 ]
Lee, Byung-Cheol [1 ]
机构
[1] Kyung Hee Univ, Coll Oriental Med, Dept Internal Med, Seoul 130702, South Korea
[2] Kyung Hee Univ, Kohwang Med Res Inst, Seoul 130702, South Korea
关键词
adipokines; glucose uptake; high-fat diet; L6 muscle cells; Platycodon grandiflorum; INSULIN-RESISTANCE; FOOD-INTAKE; TNF-ALPHA; OBESITY; LEPTIN; ADIPONECTIN; PROTEIN; HOMEOSTASIS; DISEASE; RATS;
D O I
10.1111/j.2042-7158.2012.01455.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Objectives Obesity and diabetes have become the most common human health problems worldwide. Obesity's contribution to type 2 diabetes might be due to dysregulation of adipokines and glucose uptake. Methods In this study, we performed in-vivo and in-vitro studies to evaluate the effects of Platycodon grandiflorum extract (PGE) on adipokines and glucose uptake. Before study, platycodin D concentrations were analysed by HPLC in PGE prepared in water, in 50% ethanol and in 80% ethanol, and we selected the 80% ethanol extract as the PGE for this study based on the HPLC results. Key findings We found that inclusion of PGE in the high-fat diet (HFD) markedly attenuated food intake, body weight, epididymal fat weight, adipocyte size and blood glucose levels by the oral glucose tolerance test in mice, and maintained serumlevels of adiponectin, resistin, leptin, fructosamine and triglycerides. Gene expression analysis revealed thatPGEup-regulated adiponectin, and down-regulatedTNF-aand leptin in fat tissue. InL6muscle cells in vitro, PGEincreased insulin-stimulated glucose uptake. Conclusions We conclude that PGE may improve obesity in mice fed an HFD and glucose uptake in L6 muscle cells by modifying adipokines, and could offer clinical benefits as a supplement to treat obesity and diabetes.
引用
收藏
页码:697 / 704
页数:8
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