Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disorder that affects 9-21% of reproductive-aged women. Affected women frequently display obesity, insulin resistance, and inflammation. Altered gut microbial community has been reported in PCOS and obese PCOS patients. However, the profile of the gut microbial community in insulin resistant PCOS (IR-PCOS) patients still remains unknown. In this study, next-generation sequencing based on the 16S rRNA gene was used to compare the gut microbial composition of women with IR-PCOS (n = 9, PCOS with insulin resistance), NIR-PCOS (n = 8, PCOS alone) and healthy controls (n = 8, HC). We assessed that the composition of the gut microbial communities in NIR-PCOS and IR-PCOS patients were significantly altered. The family Bacteroidaceae was prolific in the NIR-PCOS group and reached its highest level in the IR-PCOS group, while the Prevotellaceae dramatically decreased in PCOS patients, especially in the IR-PCOS group. Subsequent correlation analysis revealed that the increased clinical parameter levels, including insulin resistance, sex-hormones and inflammation, were positively associated with the abundance of Bacteroidaceae, but negatively associated with that of Prevotellaceae. In addition, IR-PCOS patients also displayed a significant difference in their amounts of Ruminococcaceae and Lachnospiraceae when compared to the NIR-PCOS group. Moreover, the functional prediction from PICRUSt revealed that 73 pathways are significantly changed in the gut microbial communities of PCOS patients. Specifically, 21 metabolism-associated pathways, including the steroid hormone biosynthesis and lipopolysaccharide biosynthesis pathways, are obviously changed in IR-PCOS when compared to NIR-PCOS and HC groups. Taking this into consideration, our present study suggests that the dysbiosis of gut microbial communities occurred most notably in IR-PCOS patients, and the difference in gut dysbiosis profile between the IR-PCOS and NIR-PCOS should be considered in clinical treatment for PCOS patients and future drugs development. (C) 2018 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
机构:
Hop Rangueil, Dept Therapeut, Toulouse, France
Fac Med Toulouse, INSERM, U558, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Amar, Jacques
Chabo, Chantal
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Chabo, Chantal
Waget, Aurelie
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Waget, Aurelie
Klopp, Pascale
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Klopp, Pascale
Vachoux, Christelle
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Vachoux, Christelle
Bermudez-Humaran, Luis G.
论文数: 0引用数: 0
h-index: 0
机构:
INRA, Unite Ecol & Physiol Syst Digestif, Jouy En Josas, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Bermudez-Humaran, Luis G.
Smirnova, Natalia
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
机构:
Univ Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Univ Colorado, Dept Anthropol, Boulder, CO 80309 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Amato, Katherine R.
Leigh, Steven R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Anthropol, Boulder, CO 80309 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Leigh, Steven R.
Kent, Angela
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Kent, Angela
Mackie, Roderick I.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Mackie, Roderick I.
Yeoman, Carl J.
论文数: 0引用数: 0
h-index: 0
机构:
Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Yeoman, Carl J.
Stumpf, Rebecca M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Anthropol, Urbana, IL USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Stumpf, Rebecca M.
Wilson, Brenda A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Microbiol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Wilson, Brenda A.
Nelson, Karen E.
论文数: 0引用数: 0
h-index: 0
机构:
J Craig Venter Inst, Rockville, MD 20850 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Nelson, Karen E.
White, Bryan A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
White, Bryan A.
Garber, Paul A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anthropol, Urbana, IL USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
机构:
Hop Rangueil, Dept Therapeut, Toulouse, France
Fac Med Toulouse, INSERM, U558, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Amar, Jacques
Chabo, Chantal
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Chabo, Chantal
Waget, Aurelie
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Waget, Aurelie
Klopp, Pascale
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Klopp, Pascale
Vachoux, Christelle
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Vachoux, Christelle
Bermudez-Humaran, Luis G.
论文数: 0引用数: 0
h-index: 0
机构:
INRA, Unite Ecol & Physiol Syst Digestif, Jouy En Josas, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
Bermudez-Humaran, Luis G.
Smirnova, Natalia
论文数: 0引用数: 0
h-index: 0
机构:
Fac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, FranceFac Med Toulouse, INSERM, U1048, Inst Rech Malad Metab & Cardiovasc Rangueil I2MC, F-31073 Toulouse, France
机构:
Univ Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Univ Colorado, Dept Anthropol, Boulder, CO 80309 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Amato, Katherine R.
Leigh, Steven R.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colorado, Dept Anthropol, Boulder, CO 80309 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Leigh, Steven R.
Kent, Angela
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Nat Resources & Environm Sci, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Kent, Angela
Mackie, Roderick I.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Mackie, Roderick I.
Yeoman, Carl J.
论文数: 0引用数: 0
h-index: 0
机构:
Montana State Univ, Dept Anim & Range Sci, Bozeman, MT 59717 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Yeoman, Carl J.
Stumpf, Rebecca M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Anthropol, Urbana, IL USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Stumpf, Rebecca M.
Wilson, Brenda A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USA
Univ Illinois, Dept Microbiol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Wilson, Brenda A.
Nelson, Karen E.
论文数: 0引用数: 0
h-index: 0
机构:
J Craig Venter Inst, Rockville, MD 20850 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
Nelson, Karen E.
White, Bryan A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anim Sci, Urbana, IL 61801 USA
Univ Illinois, Inst Genom Biol, Urbana, IL 61801 USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA
White, Bryan A.
Garber, Paul A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Illinois, Dept Anthropol, Urbana, IL USAUniv Illinois, Program Ecol Evolut & Conservat Biol, Urbana, IL 61801 USA