Simultaneous Electrochemical Speciation of Oxidized and Reduced Glutathione. Redox Profiling of Oxidative Stress in Biological Fluids with a Modified Carbon Electrode

被引:51
作者
Olmos Moya, Patricia M. [1 ]
Martinez Alfaro, Minerva [2 ]
Kazemi, Rezvan [3 ]
Apuche-Aviles, Mario A. [3 ]
Griveau, Sophie [4 ,5 ,6 ,7 ]
Bedioui, Fethi [4 ,5 ,6 ,7 ]
Gutierrez Granados, Silvia [1 ]
机构
[1] Univ Guanajuato, Dept Quim, Campus Guanajuato, Guanajuato, Mexico
[2] Univ Guanajuato, Dept Farm, Div Ciencias Nat & Exactas, Campus Guanajuato, Guanajuato, Mexico
[3] Univ Nevada, Dept Chem, Reno, NV 89557 USA
[4] PSL Res Univ, Chim ParisTech, F-75005 Paris, France
[5] INSERM, U1022, F-75005 Paris, France
[6] CNRS 8258, UTCBS, F-75005 Paris, France
[7] Univ Paris 05, Sorbonne Paris Cite, UTCBS, F-75006 Paris, France
基金
美国国家科学基金会;
关键词
COBALT PHTHALOCYANINE; ELECTROCATALYTIC ACTIVITY; LIQUID-CHROMATOGRAPHY; RATS; 2-MERCAPTOETHANOL; LEAD; METALLOPHTHALOCYANINES; NANOTUBES; LIVER; ELECTROOXIDATION;
D O I
10.1021/acs.analchem.7b01690
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The simultaneous electrochemical quantification of oxidized (GSSG) and reduced glutathione (GSH), biomarkers of oxidative stress, is demonstrated in biological fluids. The detection was accomplished by the development of a modified carbon electrode and was applied to the analysis of biological fluids of model organisms under oxidative stress caused by lead intoxication. Nanocomposite molecular material based on cobalt phthalocyanine (CoPc) and multiwalled carbon nanotubes functionalized with carboxyl groups (MWCNTO was developed to modify glassy carbon electrodes (GCE) for the detection of reduced and oxidized glutathione. The morphology of the nanocomposite film was characterized by scanning electron microscopy (SEM) and profilometry. The electrochemical behavior of the modified electrode was assessed by cyclic voltammetry (CV) to determine the surface coverage (F) by CoPc. The electrocatalytic behavior of the modified electrode toward reduced (GSH) and oxidized (GSSG) forms of glutathione was assessed by CV studies at physiological pH. The obtained results show that the combined use of CoPc and MVVCNTf results in an electro catalytic activity for GSH oxidation and GSSG reduction, enabling the simultaneous detection of both species. Differential pulse voltammetry reveals detection limits of 100 mu M for GSH and 8.3 mu M for GSSG, respectively. The potential interference from ascorbic acid, cysteine, glutamic acid, and glucose was also studied, and the obtained results show limited effects from these species. Finally, the hybrid electrode was used for the determination of GSH and GSSG in rat urine and plasma samples, intoxicated or not by lead. Both glutathione forms were detected in these complex biological matrixes without any pretreatment. Our results portray the role of GSH and GSSG as markers of oxidative stress in live organisms under lead intoxication.
引用
收藏
页码:10726 / 10733
页数:8
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