Multienzyme microbiosensor based on electropolymerized o-phenylenediamine for simultaneous in vitro determination of acetylcholine and choline

被引：38

作者：

Khan, Anish ^{[1
]}

Ab Ghani, Sulaiman ^{[1
]}

机构：

[1] Univ Sains Malaysia, Usm Pulau Pinang 11800, Malaysia

来源：

BIOSENSORS & BIOELECTRONICS | 2012年 / 31卷 / 01期

关键词：

Acetylcholine; Carbon fibre; Choline; In vitro; Poly(o-phenylenediamine); AMPEROMETRIC BIOSENSOR; ALZHEIMERS-DISEASE; BRAIN; ELECTRODE; SYSTEM; MEMORY; VIVO; DYSFUNCTION; IMPAIRMENT; STABILITY;

D O I：

10.1016/j.bios.2011.11.007

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

The electrochemical biosensors based on poly(o-phenylenediamine) (PoPD) and acetylcholinesterase (AChE) and choline oxidase (ChO) enzymes were fabricated on carbon fibre (CF) substrate. The electropolymerized PoPD was used to reduce the interfering substances. The electrode assembly was completed by depositing functionalized carbon nano tubes (FCNTs) and Nation (Naf). Amperometric detection of acetylcholine (ACh) and choline (Ch) were realized at an applied potential of +750 mV vs Ag/AgCl (saturated KCl). At pH 7.4, the final assembly, Naf-FCNTs/AChE-ChO((10:1))/PoPD/CFElip, was observed to have high sensitivity towards Ch (6.3 +/- 0.3 mu A mM(-1)) and ACh (5.8 +/- 0.3 mu A mM(-1)), linear range for Ch (K-M = 0.52 +/- 0.03 mM) and ACh (K-M = 0.59 +/- 0.07 mM), and for Ch the highest ascorbic acid blocking capacity (97.2 +/- 2 1 mM AA). It had a response time of <5 s and with 0.045 mu M limit of detection. Studies on different ratio (ACh/Ch) revealed that 10:1, gave best overall response. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：433 / 438

页数：6

共 28 条

[1] Monitoring of choline level in diabetic rat's brain during post-opioids administration by multienzyme sensors [J].

Ahmad, Farook ;

Yusof, Ahmad Pauzi Mohd ;

Bainbridge, Martina ;

Zahir, M. D. Hasan ;

Ab Ghani, Sulaiman .

SENSORS AND ACTUATORS B-CHEMICAL, 2007, 128 (01) :39-45

[2] Selective cognitive dysfunction in acetylcholine M1 muscarinic receptor mutant mice [J].

Anagnostaras, SG ;

Murphy, GG ;

Hamilton, SE ;

Mitchell, SL ;

Rahnama, NP ;

Nathanson, NM ;

Silva, AJ .

NATURE NEUROSCIENCE, 2003, 6 (01) :51-58

[3] A convergent model for cognitive dysfunctions in Parkinson's disease: the critical dopamine-acetylcholine synaptic balance [J].

Calabresi, Paolo ;

Picconi, Barbara ;

Parnetti, Lucilla ;

Di Filippo, Massimiliano .

LANCET NEUROLOGY, 2006, 5 (11) :974-983

[4] Amperometric micro-biosensor array for choline and acetylcholine based on a glassy carbon electrode modified by platinum-black particles and a co-crosslinked enzyme system [J].

Chen, J ;

Lin, XQ ;

Chen, ZH ;

Wu, SG ;

Wang, SQ .

ANALYTICAL LETTERS, 2001, 34 (04) :491-501

[5]

Curulli A, 2001, ELECTROANAL, V13, P236

[6] Synaptic plasticity and nicotine addiction [J].

Dani, JA ;

Ji, DY ;

Zhou, FM .

NEURON, 2001, 31 (03) :349-352

[7] Acetylcholine modulation of neural systems involved in learning and memory [J].

Gold, PE .

NEUROBIOLOGY OF LEARNING AND MEMORY, 2003, 80 (03) :194-210

[8]

Gouda MD, 2001, ELECTROANAL, V13, P849, DOI 10.1002/1521-4109(200106)13:10<849::AID-ELAN849>3.0.CO

[9]

2-#

[10] Electrosynthesized poly(pyrrole)/poly(2-naphthol) bilayer membrane as an effective anti-interference layer for simultaneous determination of acethylcholine and choline by a dual electrode amperometric biosensor [J].

Guerrieri, A ;

Lattanzio, V ;

Palmisano, F ;

Zambonin, PG .

BIOSENSORS & BIOELECTRONICS, 2006, 21 (09) :1710-1718

← 1 2 3 →