Fisetin Inhibits Cell Proliferation and Induces Apoptosis via JAK/STAT3 Signaling Pathways in Human Thyroid TPC 1 Cancer Cells

被引:25
作者
Liang, Ying [1 ]
Kong, Deyu [2 ]
Zhang, Yi [1 ]
Li, Siqi [1 ]
Li, Yan [1 ]
Ramamoorthy, Anuradha [3 ]
Ma, Junfeng [1 ]
机构
[1] Kunming Med Univ, Affiliated Hosp 2, Thyroid & Breast Surg, Kunming 650101, Yunnan, Peoples R China
[2] Kunming Med Univ, Affiliated Hosp 1, Internal Med Oncol, Kunming 650032, Yunnan, Peoples R China
[3] SengamalaThayaar Educ Trust Womens Coll, PG & Res Dept Biochem, Sundarakkottai, Tamil Nadu, India
关键词
fisetin; thyroid cancer; apoptosis; JAK1; STAT3; caspase; DIETARY FLAVONOID FISETIN; IN-VITRO; DEPENDENT APOPTOSIS; MITOCHONDRIAL; GROWTH; CARCINOMA; ACTIVATION; EXPRESSION; MIGRATION; PI3K/AKT;
D O I
10.1007/s12257-019-0326-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Thyroid cancer is the most important malignant tumor reported in human populations where, its treatment remains undeveloped. Fisetin, a plant flavonoid exhibits several pharmacological properties like antioxidant, anti-inflammatory, and anticancer function. In the existing study, we assessed fisetin mediated cytotoxic effects and action potential of fisetin on cell proliferation in TPC-1 human cancer cells. Also, examined the apoptosis in TPC-1 cells by reactive oxygen species (ROS) generation, the mitochondrial membrane potential (MMP) and apoptotic morphological changes through AO/EtBr staining. Additionally, we analyzed the effects of fisetin through ELISA analysis to evaluate the caspases expression and studied JAK 1 and STAT3 signaling molecule in TPC1 cells. Our results demonstrated that fisetin stimulated apoptosis, which confirmed through reduced cell viability, improved ROS generation, altered MMP and cell cycle phases in TPC-1 cells. Further, the fisetin up-regulated the expression of caspase (3, 8, and 9) expressions in TPC-1 cells. Also, we observed the fisetin down-regulated the JAK 1 and STAT3 expression in TPC1 cells. Thus, the fisetin induced apoptosis in TPC-1 cells by the induction of oxidative damage and enhanced caspases expression by down-regulating JAK 1 and STAT3 signaling molecules. Hence, the fisetin would be considered as a beneficial therapeutic agent for the thyroid cancer treatment.
引用
收藏
页码:197 / 205
页数:9
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