Association of increased CD4+ T-cell infiltration with increased IL-16 gene expression in atopic dermatitis

Background: The mechanisms involved in the initiation and the maintenance of skin inflammation in atopic dermatitis (AD) are poorly understood. Previous studies have demonstrated increased numbers of infiltrating CD4(+) T cells in acute lesions compared with normal control skin. IL-16 is a cytokine that has selective chemotactic activity for CD4(+) cells. Objective: We sought to examine whether IL-16 expression might be upregulated in acute versus chronic AD. Methods: We investigated the expression of IL-16 mRNA in skin biopsy specimens from acute and chronic skin Lesions, as well as from the uninvolved skin of patients with AD and normal skin. Cryostat sections from 4% paraformaldehyde-fixed skin biopsy specimens were processed for in situ hybridization by using cRNA coding for IL-16 mRNA, Numbers of infiltrating CD4(+) and CD8(+) cells were also determined by immunocytochemistry. Results: There were positive signals for IL-16 mRNA both in the basal layer of the epidermis and in the dermis of AD skin. biopsy specimens from all subjects studied. The numbers of epidermal and dermal IL-16 mRNA(+) cells were significantly increased in acute skin lesions compared with chronic (P<.01) and uninvolved (P<.001) skin lesions and compared with normal skin (P<.001). The number of CD4(+) cells was significantly increased in acute skin lesions compared with chronic (P<.01) skin Lesions and uninvolved skin (P<.01) and compared with normal skin (P<.01). Significant correlations were found between the numbers of CD4+ cells and the numbers of epidermal (r = 0,82, P<.001) and dermal (r = 0.71, P<.001) IL-16 mRNA+ cells. Conclusion: The results demonstrate that upregulation of IL-16 mRNA expression in acute AD is associated with increased numbers of CD4+ cells, suggesting that IL-16 may play a role in the initiation of skin inflammation, presumably through recruitment of CD4+ cells.