Enhanced weak-signal sensitivity in two-photon microscopy by adaptive illumination

被引：30

作者：

Chu, Kengyeh K. ^{[1
]}

Lim, Daryl ^{[1
]}

Mertz, Jerome ^{[1
]}

机构：

[1] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA

来源：

OPTICS LETTERS | 2007年 / 32卷 / 19期

关键词：

D O I：

10.1364/OL.32.002846

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a technique to enhance both the weak-signal relative sensitivity and the dynamic range of a laser scanning optical microscope. The technique is based on maintaining a fixed detection power by fast feedback control of the illumination power, thereby transferring high measurement resolution to weak signals while virtually eliminating the possibility of image saturation. We analyze and demonstrate the benefits of adaptive illumination in two-photon fluorescence microscopy. (c) 2007 Optical Society of America.

引用

页码：2846 / 2848

页数：3

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