Novel detection method of nitric oxide using horseradish peroxidase

被引：0

作者：

Kikuchi, K ^{[1
]}

Nagano, T ^{[1
]}

Hirobe, M ^{[1
]}

机构：

[1] UNIV TOKYO, FAC PHARMACEUT SCI, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 1996年 / 19卷 / 04期

关键词：

nitric oxide; horseradish peroxidase; Soret band; difference spectra;

D O I：

暂无

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Nitric oxide (NO) readily makes corresponding complexes not only with ferrous iron but also with ferric iron. However, NO-ferric complexes of many heme proteins were unstable, while horseradish peroxidase formed the very stable NO-ferric porphyrin complex with a shift of the Soret band of the absorption wavelength from 396.5 nm to 420.0 nm. The concentration of NO in aqueous media could be monitored by measuring the absorption changes, and the detection limit was 10 nM. The simple procedure is convenient for concentration determination of NO solution.

引用

页码：649 / 651

页数：3

共 16 条

[1] NITROSYLIRON(III) HEMOGLOBIN - AUTOREDUCTION AND SPECTROSCOPY [J].

ADDISON, AW ;

STEPHANOS, JJ .

BIOCHEMISTRY, 1986, 25 (14) :4104-4113

[2]

FELDMAN PL, 1993, CHEM ENG NEWS, V71, P26

[3] ENDOTHELIUM-DERIVED RELAXING FACTOR RELEASE ON ACTIVATION OF NMDA RECEPTORS SUGGESTS ROLE AS INTERCELLULAR MESSENGER IN THE BRAIN [J].

GARTHWAITE, J ;

CHARLES, SL ;

CHESSWILLIAMS, R .

NATURE, 1988, 336 (6197) :385-388

[4] ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].

GREEN, LC ;

WAGNER, DA ;

GLOGOWSKI, J ;

SKIPPER, PL ;

WISHNOK, JS ;

TANNENBAUM, SR .

ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138

[5] DIRECT MEASUREMENTS OF ENDOTHELIUM-DERIVED NITRIC-OXIDE RELEASE BY STIMULATION OF ENDOTHELIN RECEPTORS IN RAT-KIDNEY AND ITS ALTERATION IN SALT-INDUCED HYPERTENSION [J].

HIRATA, Y ;

HAYAKAWA, H ;

SUZUKI, E ;

KIMURA, K ;

KIKUCHI, K ;

NAGANO, T ;

HIROBE, M ;

OMATA, M .

CIRCULATION, 1995, 91 (04) :1229-1235

[6] OXIDATION OF NITRIC-OXIDE IN AQUEOUS-SOLUTION TO NITRITE BUT NOT NITRATE - COMPARISON WITH ENZYMATICALLY FORMED NITRIC-OXIDE FROM L-ARGININE [J].

IGNARRO, LJ ;

FUKUTO, JM ;

GRISCAVAGE, JM ;

ROGERS, NE ;

BYRNS, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8103-8107

[7] CONTROL OF CORONARY VASCULAR TONE BY NITRIC-OXIDE [J].

KELM, M ;

SCHRADER, J .

CIRCULATION RESEARCH, 1990, 66 (06) :1561-1575

[8] DETECTION OF NITRIC-OXIDE PRODUCTION FROM A PERFUSED ORGAN BY A LUMINOL-H2O2 SYSTEM [J].

KIKUCHI, K ;

NAGANO, T ;

HAYAKAWA, H ;

HIRATA, Y ;

HIROBE, M .

ANALYTICAL CHEMISTRY, 1993, 65 (13) :1794-1799

[9]

KIKUCHI K, 1992, CHEM PHARM BULL, V40, P2233

[10]

KIKUCHI K, 1993, J BIOL CHEM, V268, P23106

← 1 2 →