Determination of the folding of proteins as a function of denaturants, osmolytes or ligands using circular dichroism

被引：124

作者：

Greenfield, Norma J. ^{[1
]}

机构：

[1] Robert Wood Johnson Med Sch, Dept Neurosci, Piscataway, NJ 08854 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 06期

关键词：

D O I：

10.1038/nprot.2006.229

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular dichroism (CD) is an excellent tool for examining the interactions and stability of proteins. This protocol covers methods to obtain and analyze circular dichroism spectra to measure changes in the folding of proteins as a function of denaturants, osmolytes or ligands. Applications include determination of the free energy of folding of a protein, the effects of mutations on protein stability and the estimation of binding constants for the interactions of proteins with other proteins, DNA or ligands, such as substrates or inhibitors. The experiments require 2-5 h.

引用

页码：2733 / 2741

页数：9

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