Supercharging SpyCatcher toward an intrinsically disordered protein with stimuli-responsive chemical reactivity

被引：29

作者：

Cao, Yang ^{[1
]}

Liu, Dong ^{[1
]}

Zhang, Wen-Bin ^{[1
]}

机构：

[1] Peking Univ, Coll Chem & Mol Engn, Ctr Soft Matter Sci & Engn, Key Lab Polymer Chem & Phys,Minist Educ, Beijing 100871, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2017年 / 53卷 / 63期

基金：

中国国家自然科学基金;

关键词：

UNSTRUCTURED PROTEINS; GIANT MOLECULES; CLICK CHEMISTRY; ISOPEPTIDE BOND; PEPTIDE TAG; SPYTAG; STABILITY; TOPOLOGY; RESIDUES; DOMAINS;

D O I：

10.1039/c7cc04507g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report a supercharged, intrinsically disordered protein, SpyCatcher(-), possessing stimuli-responsive reactivity toward SpyTag with tunable yields ranging from 4% to 98% depending on pH, temperature, ionic strength, etc. The CD and NMR studies reveal that the reaction occurs through a folded intermediate formed probably via a different mechanism from that of SpyCatcher.

引用

收藏

页码：8830 / 8833

页数：4

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