Ex vivo expansion of human circulating myogenic progenitors on cluster-assembled nanostructured TiO2

被引：14

作者：

Belicchi, Marzia ^{[1
]}

Erratico, Silvia ^{[1
]}

Razini, Paola ^{[1
]}

Meregalli, Mirella ^{[1
]}

Cattaneo, Alessandra ^{[2
]}

Jacchetti, Emanuela ^{[4
]}

Farini, Andrea ^{[1
]}

Villa, Chiara ^{[1
]}

Bresolin, Nereo ^{[1
]}

Porretti, Laura ^{[2
]}

Lenardi, Cristina ^{[3
,5
]}

Milani, Paolo ^{[4
,5
]}

Torrente, Yvan ^{[1
]}

机构：

[1] Univ Milan, Stem Cell Lab, Fdn IRCCS Ca Granda Osped Maggiore Policlin, Ctr Dino Ferrari, I-20122 Milan, Italy

[2] Fdn IRCCS Ca Granda Osped Maggiore Policlin, Ctr Med Trasfus Terapia Cellulare & Criobiol, I-20122 Milan, Italy

[3] Univ Milan, CIMAINA, Dipartimento Sci Mol Applicate Biosistemi, I-20134 Milan, Italy

[4] Univ Milan, Dipartimento Fis, CIMAINA, I-20133 Milan, Italy

[5] Fdn Filarete, I-20139 Milan, Italy

来源：

BIOMATERIALS | 2010年 / 31卷 / 20期

关键词：

Stem cell; Titanium oxide; Nanotopography; Surface treatment; Cell culture; Muscular dystrophy; DUCHENNE MUSCULAR-DYSTROPHY; OSTEOBLAST-LIKE CELLS; MARROW STROMAL CELLS; STEM-CELLS; HEMATOPOIETIC STEM; MUSCLE; TITANIUM; EXPRESSION; ADHESION; AC133;

D O I：

10.1016/j.biomaterials.2010.03.021

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Ex vivo expansion of hematopoietic stem cells has been explored in the fields of stem cell biology, gene therapy and clinical transplantation. Recently, we demonstrated the existence of a circulating myogenic progenitor expressing the CD133 antigen. The relative inability of circulating CD133+ stem cells to reproduce themselves ex vivo imposes substantial limitations on their use for clinical applications in muscular dystrophies. Here we report that the use of cluster-assembled nanostructured titanium dioxide (ns-TiO2) substrates, in combination with cytokine enriched medium, enables high-level expansion of circulating CD133+ stem cells in vitro. Furthermore, we demonstrate that expanded circulating CD133+ stem cells retain their in vitro capacity to differentiate into myogenic cells. The exploitation of cluster-assembled ns-TiO2 substrates for the expansion of CD133+ stem cells in vitro could therefore make the clinical application of these stem cells for the treatment of muscle diseases practical. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：5385 / 5396

页数：12

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