Esterase-mediated spinosad resistance in house flies Musca domestica (Diptera: Muscidae)

被引：19

作者：

Zhang, Yi ^{[1
,2
]}

Guo, Mingcheng ^{[3
]}

Ma, Zhuo ^{[2
,4
]}

You, Chunmei ^{[2
]}

Gao, Xiwu ^{[2
]}

Shi, Xueyan ^{[2
]}

机构：

[1] Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, MOA Key Lab Pesticide Residue Detect, Inst Qual & Stand Agroprod, Hangzhou 310021, Peoples R China

[2] China Agr Univ, Dept Entomol, Beijing 100193, Peoples R China

[3] Minist Agr & Rural Affairs, Inst Control Agrochem, Beijing 100125, Peoples R China

[4] Dongcheng Ctr Dis Prevent & Control, Beijing 100009, Peoples R China

来源：

ECOTOXICOLOGY | 2020年 / 29卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Musca domestica; Spinosad resistance; Caboxylesterase; Md alpha E7; PERMETHRIN RESISTANCE; HUBNER LEPIDOPTERA; FLY; INSECTICIDE; POPULATIONS; SELECTION; STRAIN; MECHANISMS; SYNERGISM; DIAZINON;

D O I：

10.1007/s10646-019-02125-y

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Although esterase-mediated spinosad resistance has been proposed for several insects, the associated molecular mechanism remains poorly understood. In this study, we investigated the mechanism of esterase-based spinosad resistance in house flies using a susceptible strain (SSS) and a spinosad-resistant, near-isogenic line (N-SRS). Combined with the synergistic effect of DEF on spinosad in the N-SRS strain, decreased ali-esterase activity in the spinosad-resistant strain has implicated the involvement of mutant esterase in spinosad resistance in house flies. Examination of the carboxylesterase gene Md alpha E7 in the two strains revealed that four non-synonymous mutations (Trp(251)-Leu, Asp(273)-Glu, Ala(365)-Val, and Ile(396)-Val) may be associated with spinosad resistance in house flies. Single nucleotide polymorphism analysis further indicated a strong relationship between these four mutations and spinosad resistance. Moreover, quantitative real-time PCR revealed a female-linked Md alpha E7 expression pattern in the N-SRS strain, which may contribute to sex-differential spinosad resistance in house flies.

引用

页码：35 / 44

页数：10

共 41 条

[1]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[2] The same amino acid substitution in orthologous esterases confers organophosphate resistance on the house fly and a blowfly [J].