Effect of low-level Er:G laser irradiation on cultured human gingival fibroblasts

被引：112

作者：

Pourzarandian, A ^{[1
]}

Watanabe, H ^{[1
]}

Ruwanpura, SMPM ^{[1
]}

Aoki, A ^{[1
]}

Ishikawa, I ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Grad Sch, Sect Periodontol, Dept Hard Tissue Engn,Bunkyo Ku, Tokyo 1138549, Japan

来源：

JOURNAL OF PERIODONTOLOGY | 2005年 / 76卷 / 02期

关键词：

fibroblasts; gingival; lasers/therapeutic use; wound healing;

D O I：

10.1902/jop.2005.76.2.187

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Background: Low-level laser irradiation has been reported to enhance wound healing. Activation of gingival fibroblasts (GF) has a potential for early wound healing in periodontal treatment. The present study aimed to investigate the direct effect of low-level Er:YAG laser irradiation on gingival fibroblasts proliferation in order to clarify the laser effect on healing. Methods: Cultured human gingival fibroblasts (hGF) were exposed to low-power, pulsed Er:YAG laser irradiation with different energy densities ranging from 1.68 to 5.0 J/cm(2). The cultures were analyzed by means of trypan blue staining and counted under a light microscope. The effect of Er:YAG laser on hGF was also evaluated using a transmission electron microscope (TEM). Results: Cultures irradiated with Er:YAG laser presented faster cell growth when compared with untreated controls. This difference was statistically significant. Transmission electron microscopy revealed rough endoplasmic reticulum, prominent Golgi complexes, and mitochondria after laser irradiation. Conclusions: Our results showed that the low-level Er:YAG laser irradiation stimulates the proliferation of cultured gingival fibroblasts. The optimal stimulative energy density was found to be 3.37 J/cm(2). This result suggests that Er:YAG laser irradiation may be of therapeutic benefit for wound healing.

引用

页码：187 / 193

页数：7

共 49 条

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