Identification and Isolation of the Specific Allergens from Mutton by Western Blot, Chromatography and MALDI-TOF/TOF-MS

被引：1
|
作者：
Chen, Jia [1 ]
Deng, Yuanxi [1 ]
Xu, Hui [1 ]
机构：
[1] Bengbu Coll, Dept Food & Bioengn, Bengbu 233030, Peoples R China
来源：
PAKISTAN JOURNAL OF ZOOLOGY | 2018年 / 50卷 / 05期
关键词：
Specific allergen; Mutton; SDS-PAGE; Western blot; Chromatography; MALDI-TOF/TOF-MS; MASS-SPECTROMETRY; FOOD ALLERGY; AMINO-ACIDS; ANAPHYLAXIS; ACETYLTRANSFERASE; POPULATION; METABOLISM; EMERGENCY; PEPTIDES; SPECTRA;
D O I：
10.17582/journal.pjz/2018.50.5.1771.1776
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
To study the specific allergen in mutton, soluble protein from mutton was extracted, and separated by SDS-PAGE. With the positive and negative sera of rats, the special allergen was identified with the Western Blot, showing that the 12kDa was the specific allergen. The protein of 12kDa was then analyzed by ion exchange and gel filtration chromatography, and the MALDI-TOF/TOF-MS search on the Internet. The results indicate that the protein was diamine-N-acetyltransferase.
引用
收藏
页码：1771 / 1776
页数：6
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