Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

被引：159

作者：

Wu, Jianglai ^{[1
,2
,3
]}

Liang, Yajie ^{[3
]}

Chen, Shuo ^{[1
]}

Hsu, Ching-Lung ^{[3
]}

Chavarha, Mariya ^{[4
]}

Evans, Stephen W. ^{[4
]}

Shi, Dongqing ^{[4
]}

Lin, Michael Z. ^{[4
]}

Tsia, Kevin K. ^{[2
]}

Ji, Na ^{[1
,3
,5
,6
,7
]}

机构：

[1] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[2] Univ Hong Kong, Dept Elect & Elect Engn, Hong Kong, Peoples R China

[3] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[4] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[7] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA USA

来源：

NATURE METHODS | 2020年 / 17卷 / 03期

关键词：

ORIENTATION SELECTIVITY; VOLTAGE; NEURONS; SPIKES; CORTEX; MOUSE; CELLS; MICE;

D O I：

10.1038/s41592-020-0762-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice. High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.

引用

页码：287 / +

页数：6

共 50 条

[21] Label-Free Metabolic Imaging In Vivo by Two-Photon Fluorescence Lifetime Endomicroscopy
Liang, Wenxuan
Chen, Defu
Guan, Honghua
Park, Hyeon-Cheol
Li, Kaiyan
Li, Ang
Li, Ming-Jun
Gannot, Israel
Li, Xingde
ACS PHOTONICS, 2022, 9 (12) : 4017 - 4029
[22] Chronic imaging of mitochondria in the murine cerebral vasculature using in vivo two-photon microscopy
Rutkai, Ibolya
Evans, Wesley R.
Bess, Nikita
Salter-Cid, Tomas
Cikic, Sinisa
Chandra, Partha K.
Katakam, Prasad V. G.
Mostany, Ricardo
Busija, David W.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2020, 318 (06): : H1379 - H1386
[23] Multicolor Two-Photon Microscopy Imaging of Lipid Droplets and Liver Capsule Macrophages In Vivo
Kim, Eun Seo
Lee, Jeong-Mi
Kwak, Jong-Young
Lee, Hyo Won
Lee, In-Jeong
Kim, Hwan Myung
ANALYTICAL CHEMISTRY, 2024, 96 (21) : 8467 - 8473
[24] Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy
Zhang, Qinrong
Yang, Yuhan
Cao, Kevin J.
Chen, Wei
Paidi, Santosh
Xia, Chun-hong
Kramer, Richard H.
Gong, Xiaohua
Ji, Na
ELIFE, 2023, 12
[25] A Series of Carbazole Cationic Compounds with Large Two-Photon Absorption Cross Sections for Imaging Mitochondria in Living Cells with Two-Photon Fluorescence Microscopy
Liu, Xin
Sun, Yuming
Zhang, Yuanhong
Zhao, Ning
Zhao, Hongshi
Wang, Guancong
Yu, Xiaoqiang
Liu, Hong
JOURNAL OF FLUORESCENCE, 2011, 21 (02) : 497 - 506
[26] Flexible simultaneous mesoscale two-photon imaging of neural activity at high speeds
Clough, Mitchell
Chen, Ichun Anderson
Park, Seong-Wook
Ahrens, Allison M.
Stirman, Jeffrey N.
Smith, Spencer L.
Chen, Jerry L.
NATURE COMMUNICATIONS, 2021, 12 (01)
[27] In vivo two-photon microscopy of the hippocampus using glass plugs
Velasco, Mary Grace M.
Levene, Michael J.
BIOMEDICAL OPTICS EXPRESS, 2014, 5 (06): : 1700 - 1708
[28] Label-free near-infrared reflectance microscopy as a complimentary tool for two-photon fluorescence brain imaging
Mascaro, Anna Letizia Allegra
Costantini, Irene
Margoni, Emilia
Iannello, Giulio
Bria, Alessandro
Sacconi, Leonardo
Pavone, Francesco S.
BIOMEDICAL OPTICS EXPRESS, 2015, 6 (11): : 4483 - 4492
[29] Metabolic state oscillations in cerebral nuclei detected using two-photon fluorescence lifetime imaging microscopy
Zhou, Peng
Shen, Jiawei
Liang, Jun
Xue, Tian
Sun, Yuansheng
Zhang, Longhua
Tian, Changlin
CHINESE CHEMICAL LETTERS, 2023, 34 (01)
[30] Primer to Voltage Imaging With ANNINE Dyes and Two-Photon Microscopy
Kuhn, Bernd
Roome, Christopher J.
FRONTIERS IN CELLULAR NEUROSCIENCE, 2019, 13

← 1 2 3 4 5 →