Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

被引：159

作者：

Wu, Jianglai ^{[1
,2
,3
]}

Liang, Yajie ^{[3
]}

Chen, Shuo ^{[1
]}

Hsu, Ching-Lung ^{[3
]}

Chavarha, Mariya ^{[4
]}

Evans, Stephen W. ^{[4
]}

Shi, Dongqing ^{[4
]}

Lin, Michael Z. ^{[4
]}

Tsia, Kevin K. ^{[2
]}

Ji, Na ^{[1
,3
,5
,6
,7
]}

机构：

[1] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[2] Univ Hong Kong, Dept Elect & Elect Engn, Hong Kong, Peoples R China

[3] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[4] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[7] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA USA

来源：

NATURE METHODS | 2020年 / 17卷 / 03期

关键词：

ORIENTATION SELECTIVITY; VOLTAGE; NEURONS; SPIKES; CORTEX; MOUSE; CELLS; MICE;

D O I：

10.1038/s41592-020-0762-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice. High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.

引用

页码：287 / +

页数：6

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