Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

被引：159
|
作者：
Wu, Jianglai [1 ,2 ,3 ]
Liang, Yajie [3 ]
Chen, Shuo [1 ]
Hsu, Ching-Lung [3 ]
Chavarha, Mariya [4 ]
Evans, Stephen W. [4 ]
Shi, Dongqing [4 ]
Lin, Michael Z. [4 ]
Tsia, Kevin K. [2 ]
Ji, Na [1 ,3 ,5 ,6 ,7 ]
机构：
[1] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA
[2] Univ Hong Kong, Dept Elect & Elect Engn, Hong Kong, Peoples R China
[3] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA
[4] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA
[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[6] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA
[7] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA USA
来源：
NATURE METHODS | 2020年 / 17卷 / 03期
关键词：
ORIENTATION SELECTIVITY; VOLTAGE; NEURONS; SPIKES; CORTEX; MOUSE; CELLS; MICE;
D O I：
10.1038/s41592-020-0762-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice. High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.
引用
收藏
页码：287 / +
页数：6
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