Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo

被引：159

作者：

Wu, Jianglai ^{[1
,2
,3
]}

Liang, Yajie ^{[3
]}

Chen, Shuo ^{[1
]}

Hsu, Ching-Lung ^{[3
]}

Chavarha, Mariya ^{[4
]}

Evans, Stephen W. ^{[4
]}

Shi, Dongqing ^{[4
]}

Lin, Michael Z. ^{[4
]}

Tsia, Kevin K. ^{[2
]}

Ji, Na ^{[1
,3
,5
,6
,7
]}

机构：

[1] Univ Calif Berkeley, Dept Phys, Berkeley, CA 94720 USA

[2] Univ Hong Kong, Dept Elect & Elect Engn, Hong Kong, Peoples R China

[3] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA

[4] Stanford Univ, Dept Bioengn, Stanford, CA 94305 USA

[5] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

[6] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[7] Lawrence Berkeley Natl Lab, Mol Biophys & Integrated Bioimaging Div, Berkeley, CA USA

来源：

NATURE METHODS | 2020年 / 17卷 / 03期

关键词：

ORIENTATION SELECTIVITY; VOLTAGE; NEURONS; SPIKES; CORTEX; MOUSE; CELLS; MICE;

D O I：

10.1038/s41592-020-0762-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 mu m below the brain surface in head-fixed awake mice. High-speed two-photon laser scanning microscopy using a passive laser scanner based on free-space angular-chirp-enhanced delay achieves frame rates suitable for voltage imaging in vivo in the mouse brain.

引用

页码：287 / +

页数：6

共 50 条

[1] Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo
Jianglai Wu
Yajie Liang
Shuo Chen
Ching-Lung Hsu
Mariya Chavarha
Stephen W. Evans
Dongqing Shi
Michael Z. Lin
Kevin K. Tsia
Na Ji
Nature Methods, 2020, 17 : 287 - 290
[2] Ten-kilohertz two-photon microscopy imaging of single-cell dendritic activity and hemodynamics in vivo
Li, Ruijie
Wang, Sibo
Lyu, Jing
Chen, Ke
Sun, Xiaxin
Huang, Junjie
Sun, Pei
Liang, Susu
Li, Min
Yang, Mengke
Liu, Hongbang
Zeng, Shaoqun
Chen, Xiaowei
Li, Longhui
Jia, Hongbo
Zhou, Zhenqiao
NEUROPHOTONICS, 2023, 10 (02)
[3] Longitudinal In Vivo Two-Photon Fluorescence Imaging
Crowe, Sarah E.
Ellis-Davies, Graham C. R.
JOURNAL OF COMPARATIVE NEUROLOGY, 2014, 522 (08) : 1708 - 1727
[4] Video-rate hyperspectral two-photon fluorescence microscopy for in vivo imaging
Deng, Fengyuan
Ding, Changqin
Martin, Jerald C.
Scarborough, Nicole M.
Song, Zhengtian
Eakins, Gregory S.
Simpson, Garth J.
HIGH-SPEED BIOMEDICAL IMAGING AND SPECTROSCOPY III: TOWARD BIG DATA INSTRUMENTATION AND MANAGEMENT, 2018, 10505
[5] Optogenetic Imaging of Protein Activity Using Two-Photon Fluorescence Lifetime Imaging Microscopy
Murakoshi, Hideji
OPTOGENETICS: LIGHT-SENSING PROTEINS AND THEIR APPLICATIONS IN NEUROSCIENCE AND BEYOND, 2ND EDITION, 2021, 1293 : 295 - 308
[6] Two-photon fluorescence microscopy with differential aberration imaging
Chu, Kengyeh K.
Leray, Aymeric
Bifano, Thomas G.
Mertz, Jerome
MEMS ADAPTIVE OPTICS III, 2009, 7209
[7] In vivo imaging of cerebral energy metabolism with two-photon fluorescence lifetime microscopy of NADH
Yaseen, Mohammad A.
Sakadzic, Sava
Wu, Weicheng
Becker, Wolfgang
Kasischke, Karl A.
Boas, David A.
BIOMEDICAL OPTICS EXPRESS, 2013, 4 (02): : 307 - 321
[8] Two-photon fluorescence microscopy of in-vivo human skin
Hendriks, RFM
Lucassen, GW
LASER MICROSCOPY, 2000, 4164 : 116 - 121
[9] Kilohertz two-photon brain imaging in awake mice
Zhang, Tong
Hernandez, Oscar
Chrapkiewicz, Radoslaw
Shai, Adam
Wagner, Mark J.
Zhang, Yanping
Wu, Cheng-Hsun
Li, Jin Zhong
Inoue, Masatoshi
Gong, Yiyang
Ahanonu, Biafra
Zeng, Hongkui
Bito, Haruhiko
Schnitzer, Mark J.
NATURE METHODS, 2019, 16 (11) : 1119 - +
[10] Kilohertz two-photon brain imaging in awake mice
Tong Zhang
Oscar Hernandez
Radosław Chrapkiewicz
Adam Shai
Mark J. Wagner
Yanping Zhang
Cheng-Hsun Wu
Jin Zhong Li
Masatoshi Inoue
Yiyang Gong
Biafra Ahanonu
Hongkui Zeng
Haruhiko Bito
Mark J. Schnitzer
Nature Methods, 2019, 16 : 1119 - 1122

← 1 2 3 4 5 →