Synergistic efficacy of LBH and αB-crystallin through inhibiting transcriptional activities of p53 and p21

被引：8

作者：

Deng, Yun ^{[1
]}

Li, Yongqing ^{[1
]}

Fan, Xiongwei ^{[1
]}

Yuan, Wuzhou ^{[1
]}

Xie, Huaping ^{[1
]}

Mo, Xiaoyang ^{[1
]}

Yan, Yan ^{[1
]}

Zhou, Junmei ^{[1
]}

Wang, Yuequn ^{[1
]}

Ye, Xianli ^{[1
]}

Wan, Yongqi ^{[1
]}

Wu, Xiushan ^{[1
]}

机构：

[1] Hunan Normal Univ, Coll Life Sci, Key Lab MOE Dev Biol & Prot Chem, Ctr Heart Dev, Changsha 410081, Hunan, Peoples R China

来源：

BMB REPORTS | 2010年 / 43卷 / 06期

基金：

中国国家自然科学基金;

关键词：

LBH; p21; p53; Yeast two-hybrid; alpha B-crystallin; P53-DEPENDENT APOPTOSIS; SIGNALING PATHWAY; PROTEIN; EXPRESSION; HEART; GENE; CELLS; TRANSLOCATION; PROTECTION; AP-1;

D O I：

10.5483/BMBRep.2010.43.6.432

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

LBH is a transcription factor as a candidate gene for CHD associated with partial trisomy 2p syndrome. To identify potential LBH-interacting partners, a yeast two-hybrid screen using LBH as a bait was performed with a human heart cDNA library. One of the clones identified encodes alpha B-crystallin. Co-immunoprecipitation and GST pull-down assays showed that LBH interacts with alpha B-crystallin, which is further confirmed by mammalian two-hybrid assays. Co-localization analysis showed that in COS-7 cells, alpha B-crystallin that is cytoplasmic alone, accumulates partialy in the nucleus when co-transfected with LBH. Transient transfection assays indicated that overexpression of LBH or alpha B-crystallin reduced the transcriptional activities of p53 and p21, respectively, Overexpression of both alpha B-crystallin and LBH together resulted in a stronger repression of the transcriptional activities of p21 and p53. These results showed that the interaction of LBH and alpha B-crystallin may inhibit synergistically the transcriptional regulation of p53 and p21. [BMB reports 2010; 43(6): 432-437]

引用

页码：432 / 437

页数：6