Three-dimensional printed bone scaffolds: The role of nano/micro-hydroxyapatite particles on the adhesion and differentiation of human mesenchymal stem cells

被引：85
作者：
Domingos, Marco [1 ]
Gloria, Antonio [2 ]
Coelho, Jorge [3 ]
Bartolo, Paulo [1 ]
Ciurana, Joaquim [4 ]
机构：
[1] Univ Manchester, Sch Mech Aerosp & Civil Engn, Manchester M13 9PL, Lancs, England
[2] Natl Res Council Italy, Inst Polymers Composites & Biomat, Naples, Italy
[3] Univ Coimbra, Dept Chem Engn, CEMUC, Coimbra, Portugal
[4] Univ Girona, Girona, Spain
来源：
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART H-JOURNAL OF ENGINEERING IN MEDICINE | 2017年 / 231卷 / 06期
关键词：
Biomanufacturing; bioactive materials; hydroxyapatite; mesenchymal stem cells; bone tissue engineering; scaffold development; ALPHA-TRICALCIUM PHOSPHATE; IN-VITRO EVALUATION; GRAFT SUBSTITUTES; OSTEOGENIC DIFFERENTIATION; POLY(L-LACTIC ACID); OSTEOBLAST FUNCTION; TISSUE; COMPOSITE; REGENERATION; FABRICATION;
D O I：
10.1177/0954411916680236
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Bone tissue engineering is strongly dependent on the use of three-dimensional scaffolds that can act as templates to accommodate cells and support tissue ingrowth. Despite its wide application in tissue engineering research, polycaprolactone presents a very limited ability to induce adhesion, proliferation and osteogenic cell differentiation. To overcome some of these limitations, different calcium phosphates, such as hydroxyapatite and tricalcium phosphate, have been employed with relative success. This work investigates the influence of nano-hydroxyapatite and micro-hydroxyapatite (nHA and mHA, respectively) particles on the in vitro biomechanical performance of polycaprolactone/hydroxyapatite scaffolds. Morphological analysis performed with scanning electron microscopy allowed us to confirm the production of polycaprolactone/hydroxyapatite constructs with square interconnected pores of approximately 350 mu m and to assess the distribution of hydroxyapatite particles within the polymer matrix. Compression mechanical tests showed an increase in polycaprolactone compressive modulus (E) from 105.5 +/- 11.2 to 138.8 +/- 12.9MPa (PCL_nHA) and 217.2 +/- 21.8MPa (PCL_mHA). In comparison to PCL_mHA scaffolds, the addition of nano-hydroxyapatite enhanced the adhesion and viability of human mesenchymal stem cells as confirmed by Alamar Blue assay. In addition, after 14days of incubation, PCL_nHA scaffolds showed higher levels of alkaline phosphatase activity compared to polycaprolactone or PCL_mHA structures.
引用
收藏
页码：555 / 564
页数：10
相关论文
共 45 条
[1]   Design of tissue engineering scaffolds as delivery devices for mechanical and mechanically modulated signals [J].
Anderson, Eric J. ;
Knothe, Melissa L. .
TISSUE ENGINEERING, 2007, 13 (10) :2525-2538
[2]   High performance additive manufactured scaffolds for bone tissue engineering application [J].
Arafat, M. Tarik ;
Lam, Christopher X. F. ;
Ekaputra, Andrew K. ;
Wong, Siew Yee ;
He, Chaobin ;
Hutmacher, Dietmar W. ;
Li, Xu ;
Gibson, Ian .
SOFT MATTER, 2011, 7 (18) :8013-8022
[3]   Bone regeneration and stem cells [J].
Arvidson, K. ;
Abdallah, B. M. ;
Applegate, L. A. ;
Baldini, N. ;
Cenni, E. ;
Gomez-Barrena, E. ;
Granchi, D. ;
Kassem, M. ;
Konttinen, Y. T. ;
Mustafa, K. ;
Pioletti, D. P. ;
Sillat, T. ;
Finne-Wistrand, A. .
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2011, 15 (04) :718-746
[4]   BioCell Printing: Integrated automated assembly system for tissue engineering constructs [J].
Bartolo, P. ;
Domingos, M. ;
Gloria, A. ;
Ciurana, J. .
CIRP ANNALS-MANUFACTURING TECHNOLOGY, 2011, 60 (01) :271-274
[5]   Rabbit calvarial wound healing by means of seeded Caprotite® scaffolds [J].
Bidic, SMS ;
Calvert, JW ;
Marra, K ;
Kumta, P ;
Campbell, P ;
Mitchell, R ;
Wigginton, W ;
Hollinger, JO ;
Weiss, L ;
Mooney, MP .
JOURNAL OF DENTAL RESEARCH, 2003, 82 (02) :131-135
[6]   A biodegradable porous composite scaffold of PGA/β-TCP for bone tissue engineering [J].
Cao, Hong ;
Kuboyama, Noboru .
BONE, 2010, 46 (02) :386-395
[7]   Towards the Design of 3D Fiber-Deposited Poly(ε-caprolactone)/Iron-Doped Hydroxyapatite Nanocomposite Magnetic Scaffolds for Bone Regeneration [J].
De Santis, Roberto ;
Russo, Alessandro ;
Gloria, Antonio ;
D'Amora, Ugo ;
Russo, Teresa ;
Panseri, Silvia ;
Sandri, Monica ;
Tampieri, Anna ;
Marcacci, Maurilio ;
Dediu, Valentin A. ;
Wilde, Colin J. ;
Ambrosio, Luigi .
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2015, 11 (07) :1236-1246
[8]   The first systematic analysis of 3D rapid prototyped poly(ε-caprolactone) scaffolds manufactured through BioCell printing: the effect of pore size and geometry on compressive mechanical behaviour and in vitro hMSC viability [J].
Domingos, M. ;
Intranuovo, F. ;
Russo, T. ;
De Santis, R. ;
Gloria, A. ;
Ambrosio, L. ;
Ciurana, J. ;
Bartolo, P. .
BIOFABRICATION, 2013, 5 (04)
[9]   Improved osteoblast cell affinity on plasma-modified 3-D extruded PCL scaffolds [J].
Domingos, M. ;
Intranuovo, F. ;
Gloria, A. ;
Gristina, R. ;
Ambrosio, L. ;
Bartolo, P. J. ;
Favia, P. .
ACTA BIOMATERIALIA, 2013, 9 (04) :5997-6005
[10]   Evaluation of in vitro degradation of PCL scaffolds fabricated via BioExtrusion. Part 1: Influence of the degradation environment [J].
Domingos, M. ;
Chiellini, F. ;
Cometa, S. ;
De Giglio, E. ;
Grillo-Fernandes, E. ;
Bartolo, P. ;
Chiellini, E. .
VIRTUAL AND PHYSICAL PROTOTYPING, 2010, 5 (02) :65-73
12345