Aroma production by Yarrowia lipolytica in airlift and stirred tank bioreactors: Differences in yeast metabolism and morphology

被引：39

作者：

Braga, A. ^{[1
]}

Mesquita, D. P. ^{[1
]}

Amaral, A. L. ^{[1
,2
]}

Ferreira, E. C. ^{[1
]}

Belo, I. ^{[1
]}

机构：

[1] Univ Minho, CEB Ctr Biol Engn, P-4710057 Braga, Portugal

[2] Ist Politecn Coimbra, ISEC, DEQB, P-3030199 Coimbra, Portugal

来源：

BIOCHEMICAL ENGINEERING JOURNAL | 2015年 / 93卷

关键词：

Airlift bioreactor; Y; lipolytica; gamma-Decalactone; Oxygen mass transfer rate; Cellular morphology; Image analysis; OXYGEN MASS-TRANSFER; ACYL-COA OXIDASE; TRANSFER COEFFICIENT; GAMMA-DECALACTONE; IMAGE-ANALYSIS; BUBBLE-COLUMN; PREDICTION; ENHANCEMENT; ABSORPTION; STRESS;

D O I：

10.1016/j.bej.2014.09.006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of gamma-decalactone from castor oil in batch cultures of Yarrowia lipolytica W29 was compared in stirred tank (STR) and airlift bioreactors. Oxygen mass transfer from air to biphasic medium was characterized in both bioreactors through correlations for k(L)a with power input and superficial gas velocity. Different conditions of oxygen transfer rate (OTR) were selected to perform biotransformations and for both bioreactors improvement of gamma-decalactone productivity was obtained with OTR increase, with the highest value of 75 +/- 10 mg L-1 h(-1) being obtained in the STR for a k(L)a of 113 +/- 5 h(-1). However, a 2-fold increase in gamma-decalactone concentration (around 3 g) was achieved in the airlift compared to STR. Morphological characterization of the yeast cells by quantitative image analysis techniques showed that pneumatic agitation causes less impact in the cells morphology than mechanical agitation. A predominance of somewhat loose cells and quite irregular structures was observed in the STR. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：55 / 62

页数：8

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