Evaluation of charging on macromolecules in electron cryomicroscopy

被引：65
作者：
Brink, J
Sherman, MB
Berriman, J
Chiu, W
机构：
[1] Baylor Coll Med, Natl Ctr Macromol Imaging, Verna & Marrs Mclean Dept Biochem, Houston, TX 77030 USA
[2] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
来源：
ULTRAMICROSCOPY | 1998年 / 72卷 / 1-2期
关键词：
imaging charged samples; cryomicroscopy; defocused diffraction; secondary electron emission; electrical breakdown; specimen current;
D O I：
10.1016/S0304-3991(97)00126-5
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam "footprints" on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of Factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon him is included in the irradiated specimen area. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：41 / 52
页数：12
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