Microarray analysis of phosphatase gene expression in human melanoma

被引:18
作者
McArdle, L
Rafferty, MM
Satyamoorthy, K
Maelandsmo, GM
Dervan, PA
Herlyn, M
Easty, DJ [1 ]
机构
[1] Natl Univ Ireland Univ Coll Dublin, Dept Pathol, Conway Inst, Dublin 4, Ireland
[2] Wistar Inst Anat & Biol, Philadelphia, PA 19104 USA
[3] Norwegian Radium Hosp, Dept Tumor Biol, N-0310 Oslo, Norway
关键词
melanoma; protein tyrosine phosphatase; signal transduction;
D O I
10.1111/j.1365-2133.2005.06454.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Background Tyrosine phosphate is abnormally elevated in malignant melanoma, and this has been interpreted to reflect the activity of oncogenic protein tyrosine kinases. However, elevation may also arise due to decreased protein tyrosine phosphatase (PTP) expression. Objectives To survey phosphatase gene expression in melanoma cell lines, a benign naevus and normal melanocytes: we searched for downregulation of phosphatase gene expression in malignant cells that may indicate a role as melanoma suppressor genes. Methods Microarray analysis was used to compare gene expression for 133 phosphatase genes, comprising 39 PTPs, 16 dual-specificity phosphatases (DSPs), 47 serine/threonine phosphatases and 31 acid/alkaline and lipid-based phosphatases. Northern blotting analysis was used to study gene expression in human melanoma biopsies. Results There was decreased expression of four DSP genes (including PTEN); eight receptor PTP genes were downregulated in melanoma, among which were PTP-KAPPA and PTP-PI (consistent with our previous data). In addition, PTP-RF/LAR was downregulated in 13 of 22 metastatic melanomas. Conclusions The expression of multiple PTP receptors is decreased in melanoma; this may be a mechanism which stimulates autonomous growth in advanced melanoma.
引用
收藏
页码:925 / 930
页数:6
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