Hydration-aggregation pretreatment for drastically improving esterification activity of commercial lipases in non-aqueous media

被引：17

作者：

Katayama, Maho ^{[1
]}

Kuroiwa, Takashi ^{[1
]}

Suzuno, Kenya ^{[1
]}

Igusa, Ayumi ^{[1
]}

Matsui, Toru ^{[2
]}

Kanazawa, Akihiko ^{[1
]}

机构：

[1] Tokyo City Univ, Fac Engn, Setagaya Ku, 1-28-1 Tamazutsumi, Tokyo 1588557, Japan

[2] Tokyo Univ Technol, Sch Biosci & Biotechnol, Hachioji, Tokyo 1920982, Japan

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2017年 / 105卷

关键词：

Lipase-catalyzed esterification; Interfacial activation; Non-aqueous enzymology; Organic solvent; Lyophilization; INTERFACIAL ACTIVATION; CANDIDA-ANTARCTICA; ORGANIC-SOLVENTS; REVERSE MICELLES; FATTY-ACID; INFRARED-SPECTROSCOPY; ENZYMATIC CATALYSIS; HUMICOLA-LANUGINOSA; SECONDARY STRUCTURE; ESTER SYNTHESIS;

D O I：

10.1016/j.enzmictec.2017.06.007

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We investigated a novel, simple method for activating lipases in non-aqueous reaction media. Lipase powders were suspended in n-fatty alcohols and were then hydrated by adding a small amount of water. A paste-like aggregate was recovered from the mixture followed by lyophilization for obtaining activated lipases as dry powders. Lipase activity was evaluated for esterification between myristic acid and methanol in n-hexane. The activated lipases exhibited high esterification activity depending on the experiment conditions during hydration aggregation pretreatment such as the amount of added water, the temperature, the pH of added buffer solutions, and the carbon chain length of the n-fatty alcohols used as pretreatment solvents. Various commercial lipases from different origins could be activated by this method. Changes in lipase conformation induced by the hydration-aggregation pretreatment were studied based on fluorescence and Fourier-transform infrared spectroscopy.

引用

页码：30 / 37

页数：8

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