Sequential one-pot multienzyme synthesis of hyaluronan and its derivative

被引：15

作者：

Li, Shuang ^{[1
]}

Wang, Shuaishuai ^{[2
,3
]}

Fu, Xuan ^{[1
]}

Liu, Xian-wei ^{[1
]}

Wang, Peng George ^{[1
,2
,3
]}

Fang, Junqiang ^{[1
]}

机构：

[1] Shandong Univ, Natl Glycoengn Res Ctr, Shandong Prov Key Lab Glycochem Glycobiol, Jinan 250100, Shandong, Peoples R China

[2] Georgia State Univ, Dept Chem, Atlanta, GA 30302 USA

[3] Georgia State Univ, Ctr Diagnost & Therapeut, Atlanta, GA 30302 USA

来源：

CARBOHYDRATE POLYMERS | 2017年 / 178卷

基金：

中国国家自然科学基金;

关键词：

Hyaluronan; One-pot multienzyme; Sugar nucleotide; Hyaluronan derivative; Cross-linked hydrogel; 2; ACTIVE-SITES; ROOSTER COMB; CHEMICAL-MODIFICATION; ACID; SYNTHASE; PYROPHOSPHORYLASE; BIOSYNTHESIS; NUCLEOTIDE; ROUTE;

D O I：

10.1016/j.carbpol.2017.09.041

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Hyaluronan (HA) is a linear polysaccharide composed of repeating disaccharide units. It has been well documented to play an array of biological functions in cancer events. Here, we reported a sequential one-pot multienzyme (OPME) strategy for in vitro synthesis of HA and its derivatives. The strategy, which combined in situ sugar nucleotides generation with HA chain polymerization, could convert cheap monosaccharides into HA polymers without consuming exogenous sugar nucleotide donors. HA polymers (number-average molecular weight ranged from 1.5x10(4) to 5.5x 10(5) Da) with over 70% yields were efficiently synthesized and purified from this one-pot system. More importantly, partial labeled HA derivative was further synthesized by metabolic incorporation of unnatural monosaccharide analogues into the sequential OPME system. Cross-linked HA hydrogel was achieved via copper (I)-catalyzed azide-alkyne cycloaddition and exhibited novel networks consisting of both inter-and intra-connected HA chains, which could facilitate the potential applications of this unique polysaccharide.

引用

页码：221 / 227

页数：7