Mitochondrial Ca2+ uniporter is critical for store-operated Ca2+ entry-dependent breast cancer cell migration

被引：96

作者：

Tang, Shihao ^{[1
,4
]}

Wang, Xubu ^{[1
]}

Shen, Qiang ^{[2
]}

Yang, Xinyi ^{[1
]}

Yu, Changhui ^{[1
]}

Cai, Chunqing ^{[1
]}

Cai, Guoshuai ^{[3
]}

Meng, Xiaojing ^{[1
]}

Zou, Fei ^{[1
]}

机构：

[1] Southern Med Univ, Dept Occupat Hlth & Occupat Med, Sch Publ Hlth & Trop Med, Guangzhou, Guangdong, Peoples R China

[2] Univ Texas MD Anderson Canc Ctr, Dept Clin Canc Prevent, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Bioinformat & Computat Biol, Houston, TX 77030 USA

[4] Guangzhou 12 Hosp, Guangzhou, Guangdong, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2015年 / 458卷 / 01期

基金：

中国国家自然科学基金;

关键词：

MCU; Breast tumor; Migration; SOCE; TUMOR-METASTASIS; CALCIUM; INFLUX; CONTRACTION; PATHWAYS; PROTEIN;

D O I：

10.1016/j.bbrc.2015.01.092

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Metastasis of cancer cells is a complicated multistep process requiring extensive and continuous cytosolic calcium modulation. Mitochondrial Ca2+ uniporter (MW), a regulator of mitochondrial Ca2+ uptake, has been implicated in energy metabolism and various cellular signaling processes. However, whether MCU contributes to cancer cell migration has not been established. Here we examined the expression of MCU mRNA in the Oncomine database and found that MW is correlated to metastasis and invasive breast cancer. MCU inhibition by ruthenium red (RuR) or MCU silencing by siRNA abolished serumi-nduced migration in MDA-MB-231 breast cancer cells and reduced serum- or thapsigargin (TG)induced store-operated Ca2+ entry (SOCE). Serum-induced migrations in MDA-MB-231 cells were blocked by SOCE inhibitors. Our results demonstrate that MCU plays a critical role in breast cancer cell migration by regulating SOCE. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：186 / 193

页数：8

共 36 条

[1]

[Anonymous], BIOCH BIOPHYS ACTA

[2]

[Anonymous], 2020, CA Cancer J Clin, DOI DOI 10.3322/CAAC.21590

[3] Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter [J].

Baughman, Joshua M. ;

Perocchi, Fabiana ;

Girgis, Hany S. ;

Plovanich, Molly ;

Belcher-Timme, Casey A. ;

Sancak, Yasemin ;

Bao, X. Robert ;

Strittmatter, Laura ;

Goldberger, Olga ;

Bogorad, Roman L. ;

Koteliansky, Victor ;

Mootha, Vamsi K. .

NATURE, 2011, 476 (7360) :341-U111

[4] Store-Operated Ca2+ Entry [J].

Berna-Erro, Alejandro ;

Redondo, Pedro C. ;

Rosado, Juan A. .

CALCIUM SIGNALING, 2012, 740 :349-382

[5] Calcium signalling: Dynamics, homeostasis and remodelling [J].

Berridge, MJ ;

Bootman, MD ;

Roderick, HL .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2003, 4 (07) :517-529

[6] Structural and functional features and significance of the physical linkage between ER and mitochondria [J].

Csordas, Gyorgy ;

Renken, Christian ;

Varnai, Peter ;

Walter, Ludivine ;

Weaver, David ;

Buttle, Karolyn F. ;

Balla, Tamas ;

Mannella, Carmen A. ;

Hajnoczky, Gyorgy .

JOURNAL OF CELL BIOLOGY, 2006, 174 (07) :915-921

[7] Mitochondrial calcium uniporter silencing potentiates caspase-independent cell death in MDA-MB-231 breast cancer cells [J].

Curry, Merril C. ;

Peters, Amelia A. ;

Kenny, Paraic A. ;

Roberts-Thomson, Sarah J. ;

Monteith, Gregory R. .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2013, 434 (03) :695-700

[8] A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter [J].

De Stefani, Diego ;

Raffaello, Anna ;

Teardo, Enrico ;

Szabo, Ildiko ;

Rizzuto, Rosario .

NATURE, 2011, 476 (7360) :336-U104

[9] Energized mitochondria increase the dynamic range over which inositol 1,4,5-trisphosphate activates store-operated calcium influx [J].

Gilabert, JA ;

Bakowski, D ;

Parekh, AB .

EMBO JOURNAL, 2001, 20 (11) :2672-2679

[10] Store-operated Ca2+ entry depends on mitochondrial Ca2+ uptake [J].

Glitsch, MD ;

Bakowski, D ;

Parekh, AB .

EMBO JOURNAL, 2002, 21 (24) :6744-6754

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