Plasma extracellular vesicle microRNA-491-5p as diagnostic and prognostic marker for head and neck squamous cell carcinoma

被引:20
作者
Panvongsa, Wittaya [1 ,2 ]
Siripoon, Teerada [3 ]
Worakitchanon, Wittawin [2 ,4 ]
Arsa, Lalida [5 ]
Trachu, Narumol [6 ]
Jinawath, Natini [7 ,8 ]
Ngamphaiboon, Nuttapong [2 ,3 ]
Chairoungdua, Arthit [1 ,2 ,4 ]
机构
[1] Mahidol Univ, Toxicol Grad Program, Fac Sci, Bangkok, Thailand
[2] Mahidol Univ, Excellent Ctr Drug Discovery ECDD, Bangkok, Thailand
[3] Mahidol Univ, Ramathibodi Hosp, Fac Med, Dept Med,Div Med Oncol, Bangkok, Thailand
[4] Mahidol Univ, Dept Physiol, Fac Sci, 272 Rama 6 Rd, Bangkok 10400, Thailand
[5] Mahidol Univ, Ramathibodi Hosp, Fac Med, Mol Histopathol Lab,Dept Pathol, Bangkok, Thailand
[6] Mahidol Univ, Ramathibodi Hosp, Fac Med, Res Ctr, Bangkok, Thailand
[7] Mahidol Univ, Ramathibodi Hosp, Fac Med, Program Translat Med, Bangkok, Thailand
[8] Mahidol Univ, Integrat Computat BioSci Ctr ICBS, Bangkok, Nakhon Pathom, Thailand
关键词
biomarker; extracellular vesicle; head and neck squamous cell carcinoma; liquid biopsy; miRNA; OROPHARYNGEAL CANCER; HUMAN-PAPILLOMAVIRUS; SURVIVAL; EXPRESSION; INVASION;
D O I
10.1111/cas.15067
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Poor survival of patients with locally advanced head and neck squamous cell carcinoma (LA-HNSCC) is partly due to early diagnosis difficulties and the lack of reliable biomarkers for predicting treatment outcomes. In the discovery cohort, plasma-derived extracellular vesicles (EVs) from LA-HNSCC patients (n = 48) and healthy volunteers (n = 12) were used for profiling for microRNA (miRNA) expression by NanoString analysis. Ten EV-associated miRNAs were differentially expressed between LA-HNSCC patients and healthy volunteers. Subsequently, the results were validated in the individual discovery and additional cases (HNSCC, n = 73; control, n = 20) by quantitative RT-PCR. Among 10 EV-miRNAs, four (miR-27b-3p, miR-491-5p, miR-1910-5p, and miR-630) were significantly dysregulated in LA-HNSCC patients (n = 73) compared with healthy volunteers (n = 20). The miRNA prediction models were developed to discriminate HNSCC patients from healthy volunteers. The model using miR-491-5p was selected as a diagnostic biomarker for LA-HNSCC with a sensitivity and specificity of 46.6% and 100%, respectively (P < .001). The dynamic changes of miRNA model score (Delta miRNAs) were determined using scores pre- and postdefinitive treatment to further investigate the prognostic value of miRNA prediction models. The univariate and multivariate analyses indicated that Delta miR-491-5p was the most powerful and independent prognostic indicator for overall survival (hazard ratio [HR] 5.66, 95% confidence interval, 1.77-18.01; P = .003) and disease-free survival (HR 2.82, 95% CI, 1.13-7.05; P = .027) of HNSCC patients. In summary, the miR-491-5p prediction model could serve as a blood-based diagnostic marker for LA-HNSCC. Moreover, Delta miR-491-5p could be a potential monitoring prognostic marker to reflect the survival of HNSCC patients.
引用
收藏
页码:4257 / 4269
页数:13
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