Random amplified polymorphic DNA analysis detects variation in a micro propagated clone of Trichodesma indicum (L.) R. Br.

RAPD profile revealed variation among the individuals of a single clone of micro propagated plants of Trichodesma indicum. Callus was developed from a single zygotic embryo differentiated into multiple micro shoots on Murashige and Skoog's medium supplemented with 2.32 mu M kinetin. A single node excised from a micro shoot produced multiple shoots after three sub cultures on MS medium containing 0.23 mu M kinetin. Morphologically, all the in vitro raised plantlets appeared alike except size variation. Random amplified polymorphic DNA ( RAPD) profile of a clone of 10 plantlets prepared with MAP 1 - 20 primers amplified 119 bands: 117 monomorphic and 2 polymorphic. Perhaps, variation occurred during subculture of nodal segments rather than differentiation of shoots from callus.