UDP-glucose dehydrogenase from Capra hircus liver: Purification, partial characterization and evaluation as a coupling enzyme in UDP-galactose 4-epimerase assay

被引：5

作者：

Banerjee, Nupur ^{[1
]}

Bhattacharyya, Debasish ^{[1
]}

机构：

[1] CSIR, Indian Inst Chem Biol, Struct Biol & Bioinformat Div, Kolkata 700032, India

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2011年 / 68卷 / 01期

关键词：

UDP-glucose dehydrogenase; Caprine liver; Enzyme purification; UDP-galactose; 4-epimerase; Coupled enzyme assay; Enzyme stability; URIDINE DIPHOSPHOGLUCOSE DEHYDROGENASE; ESCHERICHIA-COLI; KLUYVEROMYCES-FRAGILIS; CDNA CLONING; RAT-LIVER; BIOSYNTHESIS; EXPRESSION; ENCODES; DIPHOSPHOGALACTOSE; HYALURONAN;

D O I：

10.1016/j.molcatb.2010.09.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

UDP-glucose dehydrogenase from Capra Inrcus has been purified to homogeneity by salt fractionations, heat treatment and chromatographic steps It is a homohexamer of about 300 kDa Though the basic physical and enzymatic properties of the caprine enzyme are comparable to those of the beef liver enzyme it has lower energy of activation and different entropy and enthalpy for the transition state during catalysis The caprine enzyme can act suitably as an auxiliary enzyme in the coupled assay system for UDP-galactose 4-epimerase Enzymes UDP-Glc DH UDP-glucose dehydrogenase (EC 111 22) Epimerase UDP-galactose 4-epimerase (EC 5 1 3 2) (c) 2010 Elsevier BV All rights reserved

引用

页码：37 / 43

页数：7

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