In vivo and in vitro effects of FSH on oocyte maturation and developmental competence

There is increasing evidence demonstrating that oocyte quality depends on the events that occur before germinal vesicle breakdown (GVBD), suggesting that the oocyte must accumulate the appropriate information for meiotic resumption fertilization and early embryonic development before chromosome condensation. This situation seems to prevail in large mammals and particularly in the bovine where we have more information than in other species. Signaling events at two different levels controls the changes that must take place for follicular growth and attainment of oocyte developmental competence. The first signaling event comes from the proper differentiation of the follicle as it normally occurs in the dominant follicle in preparation for ovulation. The second signaling event occurs as the process of follicle differentiation signals directly to the oocyte, possibly through the cumulus cells, that conditions are suitable for further embryo development. The first signal, follicular differentiation, becomes possible though a rise and fall of FSH in the circulation, while the second signal might be mimicked partially by the same hormone acting on the cumulus cells. Although FSH is likely involved in these two signaling events, the processes involved are quite different and analysis of gene expression in granulosa, cumulus and oocyte is starting to reveal the complexity of this system. The next challenge is to combine these two pathways into a functional signaling cascade. To be successful and obtain meaningful information, these genomic analyses must be developed and performed in precisely defined conditions of follicular growth and differentiation or culture conditions. Functional genomics already started with the study of function of several genes and genes families in the regulation of follicular growth and follicle-oocyte co-differentiation (i.e. IGF and BNIP genes families, EGF). (C) 2007 Published by Elsevier Inc.