Role of miR-34c in ketamine-induced neurotoxicity in neonatal mice hippocampus

被引：40

作者：

Cao, Shu-e ^{[1
]}

Tian, Jianmin ^{[1
]}

Chen, Shengyang ^{[1
]}

Zhang, Xiaoran ^{[1
]}

Zhang, Yongqiang ^{[1
]}

机构：

[1] XinXiang Med Coll, Affiliated Hosp 1, Dept Anesthesiol, Weihui 453100, HeNan Province, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2015年 / 39卷 / 02期

关键词：

hippocampus; ketamine; miR-34c; neurotoxicity; DEVELOPING BRAIN; MICRORNA; NEURODEGENERATION; EXPRESSION; NEURONS; PATHWAY; BCL-2;

D O I：

10.1002/cbin.10349

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Ketamine is a commonly used pediatric anesthetic, but it might affect development, or even induce neurotoxicity in the neonatal brain. We have used an in vivo neonatal mouse model to induce ketamine-related neurotoxicity in the hippocampus, and found that miR-34c, a microRNA associated with pathogenesis of Alzheimer's disease, was significantly upregulated during ketamine-induced hippocampal neurodegeneration. Functional assay of silencing miR-34c demonstrated that downregulation of miR-34c activated PKC-ERK pathway, upregulated anti-apoptotic protein BCL2, and ameliorated ketamine-induced apoptosis in the hippocampus. Cognitive examination with the Morris water maze test showed that ketamine-induced memory impairment was significantly improved by miR-34c downregulation. Thus, miR-34c is important in regulating ketamine-induced neurotoxicity in hippocampus.

引用

页码：164 / 168

页数：5

共 24 条

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