lncRNA GHET1 knockdown suppresses breast cancer activity in vitro and in vivo

被引：2

作者：

Han, Mingli ^{[1
,2
]}

Wang, Yimeng ^{[3
]}

Gu, Yuanting ^{[1
]}

Ge, Xin ^{[1
]}

Seng, Jingjing ^{[1
]}

Guo, Guangcheng ^{[1
]}

Zhang, Xiangyu ^{[1
]}

Zhao, Yang ^{[1
]}

Dou, Dongwei ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Affiliated Hosp 1, Dept Breast Surg, Zhengzhou 450052, Henan, Peoples R China

[2] Zhengzhou Univ, Affiliated Hosp 1, Key Lab, Zhengzhou 450052, Henan, Peoples R China

[3] Zhengzhou Univ, Affiliated Hosp 1, Dept Geriatr Endocrinol, Zhengzhou 450052, Henan, Peoples R China

来源：

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH | 2019年 / 11卷 / 01期

关键词：

GHET1; EGFR; proliferation; apoptosis; migration; invasion; MCF-7; INHIBITS CELL-PROLIFERATION; C-MYC; INVASION; PROMOTES; APOPTOSIS; ACTIVATION; MIGRATION; RESISTANCE; HOTAIR;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Long non-coding RNA gastric carcinoma high-expressed transcript 1 (lncRNA GHET1) is highly expressed in many tumors. The aim of the present study was to determine whether GHET1 inhibition decreases growth and metastasis of MCF-7 breast cancer cells by modulating epidermal growth factor receptor (EGFR) expression. In vitro, lncRNAGHET1 knockdown suppressed cell proliferation, migration, and invasion and enhanced cell apoptosis by maintaining MCF-7 cells in the G1 phase of the cell cycle. Furthermore, lncRNA GHET1 knockdown reduced the expression of EGFR and related proteins. Treatment of mice with a GHET1 inhibitor prevented tumor growth in vivo. The results indicate that lncRNA GHET1 inhibition directly suppresses EGFR expression, significantly inhibiting the downstream PI3K/AKT/Cyclin D1/MMP2/9 pathway. This mechanism may underlie the suppression of breast cancer cell activities including proliferation, migration, and invasion. In conclusion, lncRNA GHET1 knockdown suppresses tumor growth and metastasis by suppressing the activity of EGFR and downstream pathways.

引用

页码：31 / 44

页数：14

共 50 条

[31] LncRNA GHET1 predicts poor prognosis in hepatocellular carcinoma and promotes cell proliferation by silencing KLF2 [J].

Jin, Li ;

He, Yangke ;

Tang, Shijia ;

Huang, Shan .

JOURNAL OF CELLULAR PHYSIOLOGY, 2018, 233 (06) :4726-4734

[32] Novel STAT3 Inhibitor Exerts Anti-breast Cancer Effects Both in vitro and in vivo [J].

Liu, Zhe ;

Sun, Yiming ;

Yu, Meiling ;

Huang, Yingying ;

Ma, Liang ;

Kong, Lingti .

LETTERS IN DRUG DESIGN & DISCOVERY, 2023, 20 (12) :2070-2079

[33] EphB4 knockdown suppresses renal cancer cells growth and inhibits the activity of ERK and STAT3 in vitro and in vivo [J].

Zhang, Yawei ;

Chen, Lin ;

Hu, Weifeng ;

Guo, Yonglian ;

Li, Guohao ;

Chang, Lei .

INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2019, 12 (04) :3686-3693

[34] Knockdown of lncRNA PCAT1 Enhances Radiosensitivity of Cervical Cancer by Regulating miR-128/GOLM1 Axis [J].

Ge, Xingping ;

Gu, Yongfei ;

Li, Dianzu ;

Jiang, Maozhu ;

Zhao, Shuliang ;

Li, Zhengliang ;

Liu, Shuliang .

ONCOTARGETS AND THERAPY, 2020, 13 :10373-10385

[35] Expression and clinical significance of long-non-coding RNA GHET1 in pancreatic cancer [J].

Zhou, H. -Y. ;

Zhu, H. ;

Wu, X. -Y. ;

Chen, X. -D. ;

Oiao, Z. -G. ;

Ling, X. ;

Yao, X. -M. ;

Tang, J. -H. .

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES, 2017, 21 (22) :5081-5088

[36] Knockdown of FOXK1 suppresses liver cancer cell viability by inhibiting glycolysis [J].

Cui, Hong ;

Gao, Qinqin ;

Zhang, Ling ;

Han, Feng ;

Wang, Li .

LIFE SCIENCES, 2018, 213 :66-73

[37] Long non-coding RNA GHET1 promotes thyroid cancer cell proliferation and invasion [J].

Liu, Yan ;

Shi, Ping ;

Fang, Hao ;

Zhao, Zhen ;

Yang, Fei ;

Zhang, Jie ;

Jing, Shanghua ;

Geng, Cuizhi .

TRANSLATIONAL CANCER RESEARCH, 2021, 10 (09) :4148-4157

[38] Knockdown of lncRNA PCAT6 Enhances Radiosensitivity in Triple-Negative Breast Cancer Cells by Regulating miR-185-5p/TPD52 Axis [J].

Shi, Rui ;

Wu, Peng ;

Liu, Miaomiao ;

Chen, Bing ;

Cong, Longjiao .

ONCOTARGETS AND THERAPY, 2020, 13 :3025-3037

[39] RETRACTED: Targeting of GIT1 by miR-149*in breast cancer suppresses cell proliferation and metastasis in vitro and tumor growth in vivo (Retracted Article) [J].

Dong, Yan ;

Chang, Cai ;

Liu, Jingtian ;

Qiang, Jinwei .

ONCOTARGETS AND THERAPY, 2017, 10 :5873-5882

[40] lncRNA SNHG1 suppresses gastric cancer cell proliferation and promotes apoptosis via Notch1 pathway [J].

Zhang, Zhu ;

Wang, Huahong .

JOURNAL OF BUON, 2020, 25 (01) :302-307

← 1 2 3 4 5 →