L1 is Highly Expressed in Tumors of the Nervous System: A Study of Over 8000 Human Tissues

Background. L1 cell adhesion molecule (CD171) has beendetected in differentmalignant tumors and is associated with unfavorable outcome. It thus represents a target for tumor diagnosis and therapy. In this study, weassessed L1 expression inmore than 8000 normal human tissues and different types of tumors, both malignant and non-malignant, and neural and non-neural. Materials and Methods. Tissue micro-arrays, including a multi-tumor-array of 128 different tumor types, up to 50 samples of each type (approximately 5500 different samples), arrays with approximately 3000 different prostate and 600 mesenchymal tumor samples, and a normal human tissue-array were analyzed by immunohistochemistry with a monoclonal antibody using immunoperoxidase staining. Results. L1 expression was detected in tumors of neural and neural crest origin and other types of non-neural tumors, but not in those of epithelial origin. In normal human tissues, L1 was detected in skin basal cells and small blood vessels, most notably in the mature placenta and peripheral nerves. Conclusion. This first comprehensive study of the importance of L1 expression in human demonstrates strong L1 overexpression in tumors of neuroectodermal and neural crest origin and an expression in only very few normal human tissues. L1 thus is a potentially important therapeutic target, particularly with respect to malignant melanoma, gastrointestinal stromal tumor, neuroblastoma, and certain subtypes of nonneural tumors. (C) 2012 Elsevier Inc. All rights reserved.