Antioxidant flavonoids and chlorogenic acid from the leaves of Eriobotrya japonica

被引：169

作者：

Jung, HA

Park, JC

Chung, HY

Kim, J

Choi, JS ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Food & Life Sci, Pusan 608737, South Korea

[2] Sunchon Natl Univ, Dept Oriental Med Resources, Sunchon 540742, South Korea

[3] Pusan Natl Univ, Dept Pharm, Pusan 609735, South Korea

[4] Masan Coll, Masan 630729, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 1999年 / 22卷 / 02期

关键词：

Eriobotrya japonica; antioxidant activity; chlorogenic acid; flavonoids;

D O I：

10.1007/BF02976549

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DP PH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at 37 degrees C, using 2-thiobarbituric acid (TBA). The methanol extract and its tractions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCI gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH traction, and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and 2 alpha-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

引用

页码：213 / 218

页数：6

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