Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, chicken and turkey

被引：77

作者：

Koeppel, Rene ^{[1
]}

Ruf, Jueg ^{[2
]}

Zimmerli, Franziska ^{[1
]}

Breitenmoser, Alda ^{[1
]}

机构：

[1] Offcial Food Control Author Canton Zurich, Zurich, Switzerland

[2] Offcial Food Control Author Canton Thurgau, Zurich, Switzerland

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2008年 / 227卷 / 04期

关键词：

multiplex; real-time quantitative PCR; beef; pork; chicken; turkey;

D O I：

10.1007/s00217-008-0837-7

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Many meat products are composed of two or more meat species. To determine the proportion of these meat fractions, a quantitative multiplex PCR was developed for the quantification of beef, pork, chicken and turkey. This system proved its applicability, precision and accuracy in examining different meat products from the market. Thus it allows the efficient control of composed meat products in official food control and production control laboratories.

引用

页码：1199 / 1203

页数：5

共 14 条

[1]

Binke R., 2005, Mitteilungsblatt der Fleischforschung Kulmbach, V44, P201

[2] Sensitive and semi-quantitative TaqMan™ real-time polymerase chain reaction systems for the detection of beef (Bos taurus) and the detection of the family Mammalia in food and feed [J].

Brodmann, PD ;

Moore, D .

MEAT SCIENCE, 2003, 65 (01) :599-607

[3] Quantitative PCR detection of pork in raw and heated ground beef and pate [J].

Calvo, JH ;

Osta, R ;

Zaragoza, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2002, 50 (19) :5265-5267

[4]

EUGSTER AA, EUR FOOD RE IN PRESS

[5] The detection of commercial duck species in food using a single probe-multiple species-specific primer real-time PCR assay [J].

Hird, H ;

Chisholm, J ;

Brown, J .

EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2005, 221 (3-4) :559-563

[6] Nachweisverfahren für Rindfleisch in Lebensmitteln unter Anwendung der TaqManTM-Technologie [J].

I. Laube ;

A. Butschke ;

J. Zagon ;

A. Spiegelberg ;

M. Schauzu ;

K.-W. Bögl ;

L.W. Kroh ;

H. Broll .

Bundesgesundheitsblatt - Gesundheitsforschung - Gesundheitsschutz, 2001, 44 (4) :326-330

[7] Methods for the detection of beef and pork in foods using real-time polymerase chain reaction [J].

Laube, I ;

Spiegelberg, A ;

Butschke, A ;

Zagon, J ;

Schauzu, M ;

Kroh, L ;

Broll, H .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2003, 38 (02) :111-118

[8] Quantitative determination of commercially relevant species in foods by real-time PCR [J].

Laube, Ines ;

Zagon, Jutta ;

Broll, Hermann .

INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2007, 42 (03) :336-341

[9] Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction [J].

López-Andreo, M ;

Lugo, L ;

Garrido-Pertierra, A ;

Prieto, MI ;

Puyet, A .

ANALYTICAL BIOCHEMISTRY, 2005, 339 (01) :73-82

[10] A quick and simple method for the identification of meat species and meat products by PCR assay [J].

Matsunaga, T ;

Chikuni, K ;

Tanabe, R ;

Muroya, S ;

Shibata, K ;

Yamada, J ;

Shinmura, Y .

MEAT SCIENCE, 1999, 51 (02) :143-148

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