Osteogenesis-promoting Effects of the Electrospun Nanofibers Containing Decellularized Bone Matrix

Porcine decellularized bone matrix ( DBM ) was firstly prepared, and then solubilized via pepsin digestion. Solubilized DBM and poly ( L-lactic acid ) ( PLLA ) were subsequently processed into PLLA/DBM electrospun nanofibers through eletrospinning technology. The morphology , wettability and mineralization ability of PLLA/DBM electrospun nanofibers were examined, and cytocompatibility and osteogenic differentiation capacity were evaluated by culturing bone marrow mesenchymal cells ( BMSCs ) on PLLA/DBM electrospun nanofibers. The results showed that cellular components in cancellous bone could be effectively removed after decellularization, as evidenced by the significant decrease in DNA content. The solubilized DBM could be dissolved in 1, 1, 1, 3, 3 , 3-hexafluoro-2-propanol ( HFIP ) for electrospinning of the PLLA/DBM composite nanofibers formulated at different mass ratios ( 10 : 0 , 9 1, 7 3 and 5 5 ). The obtained PLLA/DBM electrospun nanofibers were demonstrated to be hydrophilic and cytocompatible , and significantly promoted the attachment and osteogenic differentiation of the BMSCs. Furthermore, the presence of DBM facilitated mineral deposition on the nanofiber surface in simulated body fluid ( SBF ) , which suggests its great potential in promoting bonding with the natural bone tissue.