We have investigated the transport and canalicular enrichment of fluorescent phosphatidylcholine (PC) in HepG2 cells using the fluorescent analogs of PC C6-NBD-PC and beta -BODIPY-PC. Fluorescent PC was efficiently transported to the biliary canaliculus (BC) and became enriched on the lumenal side of the canalicular membrane as shown for C6-NBD-PC. Some fluorescent PC was transported in vesicles to a subapical compartment (SAC) or apical recycling compartment (ARC) in polarized HepG2 cells as shown by colocalization with fluorescent sphingomyelin (C6-NBD-SM) and fluorescent transferrin, respectively. Extensive trafficking of vesicles containing fluorescent PC between the basolateral domain, the SAC/ARC and the BC as well as endocytosis of PC analogs from the canalicular membrane were found. Evidence for nonvesicular transport included enrichment of the PC-analog beta -BODIPY-PC in the BC (t(1/2)=3.54 min) prior to its accumulation in the SAC/ARC (t(1/2)=18.5 min) at 37 degreesC. Transport of fluorescent PC to the canalicular membrane also continued after disruption of the actin or microtubule cytoskeleton and at 2 degreesC. These results indicate that: (i) a nonvesicular transport pathway significantly contributes to the canalicular enrichment of PC in hepatocytic cells, and (ii) vesicular transport of fluorescent PC occurs from both membrane domains via the SAC/ARC.
机构:
Third Mil Med Univ, Dept Microbiol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Xu, Xiao-Feng
Chen, Zong-Tao
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Third Mil Med Univ, Dept Microbiol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Chen, Zong-Tao
Zhang, Jun-Lei
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Third Mil Med Univ, Dept Microbiol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Zhang, Jun-Lei
Chen, Wei
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Third Mil Med Univ, Dept Microbiol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Chen, Wei
Wang, Jia-Li
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Third Mil Med Univ, Dept Microbiol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Wang, Jia-Li
Tian, Yan-Ping
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Third Mil Med Univ, Dept Histol & Embryol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Tian, Yan-Ping
Gao, Na
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Third Mil Med Univ, Dept Histol & Embryol, Chongqing, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
Gao, Na
An, Jing
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Capital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R ChinaCapital Med Univ, Sch Basic Med Sci, Dept Microbiol, Beijing 100069, Peoples R China
机构:
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Rongrong Huang
Zhongsi Chen
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Zhongsi Chen
Mei Liu
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Mei Liu
Yan Deng
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of TechnologyState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Yan Deng
Song Li
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast UniversityState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Song Li
Nongyue He
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State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of TechnologyState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University
机构:
Jilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R ChinaJilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R China
Song, Mingjie
Bao, Haiying
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Jilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R ChinaJilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R China
Bao, Haiying
Bau, Tolgor
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Jilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R ChinaJilin Agr Univ, Coll Tradit Chinese Med Mat, 2888 Xincheng St, Changchun 130118, Jilin, Peoples R China