Vesicular and nonvesicular transport of phosphatidylcholine in polarized HepG2 cells

被引:35
作者
Wüstner, D
Mukherjee, S
Maxfield, FR
Müller, P
Hermann, A
机构
[1] Humboldt Univ, Math Nat Wissensch Fak 1, Inst Biol Biophys, D-10115 Berlin, Germany
[2] Cornell Univ, Weill Med Coll, Dept Biochem, New York, NY 10021 USA
关键词
fluorescence microscopy; fluorescent phospholipids; HepG2; cells; lipids; phosphatidylcholine; transport;
D O I
10.1034/j.1600-0854.2001.9o135.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have investigated the transport and canalicular enrichment of fluorescent phosphatidylcholine (PC) in HepG2 cells using the fluorescent analogs of PC C6-NBD-PC and beta -BODIPY-PC. Fluorescent PC was efficiently transported to the biliary canaliculus (BC) and became enriched on the lumenal side of the canalicular membrane as shown for C6-NBD-PC. Some fluorescent PC was transported in vesicles to a subapical compartment (SAC) or apical recycling compartment (ARC) in polarized HepG2 cells as shown by colocalization with fluorescent sphingomyelin (C6-NBD-SM) and fluorescent transferrin, respectively. Extensive trafficking of vesicles containing fluorescent PC between the basolateral domain, the SAC/ARC and the BC as well as endocytosis of PC analogs from the canalicular membrane were found. Evidence for nonvesicular transport included enrichment of the PC-analog beta -BODIPY-PC in the BC (t(1/2)=3.54 min) prior to its accumulation in the SAC/ARC (t(1/2)=18.5 min) at 37 degreesC. Transport of fluorescent PC to the canalicular membrane also continued after disruption of the actin or microtubule cytoskeleton and at 2 degreesC. These results indicate that: (i) a nonvesicular transport pathway significantly contributes to the canalicular enrichment of PC in hepatocytic cells, and (ii) vesicular transport of fluorescent PC occurs from both membrane domains via the SAC/ARC.
引用
收藏
页码:277 / 296
页数:20
相关论文
共 50 条
  • [1] Hexadecylphosphocholine alters nonvesicular cholesterol traffic from the plasma membrane to the endoplasmic reticulum and inhibits the synthesis of sphingomyelin in HepG2 cells
    Marco, Carmen
    Jimenez-Lopez, Jose M.
    Rios-Marco, Pablo
    Segovia, Josefa L.
    Carrasco, Maria P.
    INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2009, 41 (06) : 1296 - 1303
  • [2] Hexadecylphosphocholine interferes with the intracellular transport of cholesterol in HepG2 cells
    Carrasco, Maria P.
    Jimenez-Lopez, Jose M.
    Segovia, Josefa L.
    Marco, Carmen
    FEBS JOURNAL, 2008, 275 (08) : 1675 - 1686
  • [3] Analysis of Polarized Secretion of Fucosylated Alpha-Fetoprotein in HepG2 Cells
    Nakagawa, Tsutomu
    Moriwaki, Kenta
    Terao, Naoko
    Nakagawa, Takatoshi
    Miyamoto, Yasuhide
    Kamada, Yoshihiro
    Miyoshi, Eiji
    JOURNAL OF PROTEOME RESEARCH, 2012, 11 (05) : 2798 - 2806
  • [4] Curcumin Effect on Copper Transport in HepG2 Cells
    Berzina, Anita
    Martinsone, Inese
    Svirskis, Simons
    Murovska, Modra
    Kalis, Martins
    MEDICINA-LITHUANIA, 2018, 54 (02):
  • [5] Influence of deoxynivalenol on the oxidative status of HepG2 cells
    Bodea, G. O. Dragomir
    Munteanu, M. C.
    Dinu, D.
    Serban, A. I.
    Roming, F. Israel
    Costache, M.
    Dinischiotu, A.
    ROMANIAN BIOTECHNOLOGICAL LETTERS, 2009, 14 (02): : 4349 - 4359
  • [6] Lipid Efflux Mediated by Alkylphospholipids in HepG2 Cells
    Rios-Marco, Pablo
    Segovia, Josefa L.
    Jimenez-Lopez, Jose M.
    Marco, Carmen
    Carrasco, Maria P.
    CELL BIOCHEMISTRY AND BIOPHYSICS, 2013, 66 (03) : 737 - 746
  • [7] Rab8, a vesicular traffic regulator, is involved in dengue virus infection in HepG2 cells
    Xu, Xiao-Feng
    Chen, Zong-Tao
    Zhang, Jun-Lei
    Chen, Wei
    Wang, Jia-Li
    Tian, Yan-Ping
    Gao, Na
    An, Jing
    INTERVIROLOGY, 2008, 51 (03) : 182 - 188
  • [8] Differences in Transport Characteristics and Cytotoxicity of Epirubicin and Doxorubicin in HepG2 and A549 Cells
    Nagai, Katsuhito
    Fukuno, Shuhei
    Shiota, Maho
    Tamura, Mayuko
    Yabumoto, Sayaka
    Konishi, Hiroki
    ANTICANCER RESEARCH, 2021, 41 (12) : 6105 - 6112
  • [9] The aptamers generated from HepG2 cells
    Rongrong Huang
    Zhongsi Chen
    Mei Liu
    Yan Deng
    Song Li
    Nongyue He
    Science China(Chemistry), 2017, (06) : 786 - 792
  • [10] FPOA induces the apoptosis of HepG2 cells
    Song, Mingjie
    Bao, Haiying
    Bau, Tolgor
    EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2018, 15 (03) : 2649 - 2654