Contribution of Opioid Receptors to the Cytoprotective Effect of the Adaptation to Chronic Hypoxia at Anoxia/Reoxygenation of Isolated Cardiomyocytes

被引：6

作者：

Naryzhnaya, N. V. ^{[1
,2
]}

Maslov, L. N. ^{[1
,2
]}

Prokudina, E. S. ^{[1
]}

Lishmanov, Yu. B. ^{[1
,2
]}

机构：

[1] Russian Acad Med Sci, Cardiol Res Inst, Siberian Div, Tomsk, Russia

[2] Natl Res Tomsk Polytech Univ, Tomsk, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2015年 / 159卷 / 02期

关键词：

cardiomyocytes; hypoxia; reoxygenation; adaptation; opioids; K-ATP CHANNELS; ACTIVATION; PEPTIDES; RABBITS; KINASE; RATS;

D O I：

10.1007/s10517-015-2924-7

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We examined the role of opioid receptors in the cardioprotective effect of chronic continuous normobaric hypoxia in the model of anoxia/reoxygenation of isolated cardiomyocytes. Adaptation to hypoxia was provided by placing the rats for 21 days in an atmosphere with low O-2 content. In intact rats, anoxia/reoxygenation of cardiomyocytes caused death of 23% cells and increased lactate dehydrogenase release from cardiomyocytes. In adapted rats, anoxia/ reoxygenation of cardiomyocytes caused death of only 2.5% cells and the release of lactate dehydrogenase decreased by 25%. Preincubation of the cells with opioid receptor blocker naloxone (300 nM) for 25 min eliminated the adaptive decrease in cell survival and reduction in lactate dehydrogenase release. Hence, opioid receptors of the cardiomyocytes contribute to the cytoprotective effect of chronic normobaric hypoxia.

引用

页码：209 / 212

页数：4

共 50 条

[31] The cytoprotective effect of Gymnema inodorum leaf extract against hypoxia-induced cardiomyocytes injury
Surinkaew, Sirirat
Sun, Dali
Kooltheat, Nateelak
Boonhok, Rachasak
Somsak, Voravuth
Kumphune, Sarawut
HELIYON, 2024, 10 (15)
[32] Protective Effect of Paeoniflorin on Hypoxia Reoxygenation Cardiomyocytes and its Mechanism Based on MAPK Signal Pathway
Qin, Shu-Zhi
Ling, Wen -Pei
Yin, Mei-Fang
Luo, Chun -Yu
Zhao, Cheng-Guo
PAKISTAN JOURNAL OF ZOOLOGY, 2024, 56 (01) : 157 - 163
[33] Met5-enkephalin protects isolated adult rabbit cardiomyocytes via δ-opioid receptors
Takasaki, Y
Wolff, RA
Chien, GL
Van Winkle, DM
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 1999, 277 (06): : H2442 - H2450
[34] Overexpression of the NHE1 isoform of the Na+/H+ exchanger causes elevated apoptosis in isolated cardiomyocytes after hypoxia/reoxygenation challenge
Karki, Pratap
Fliegel, Larry
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2010, 338 (1-2) : 47 - 57
[35] Influence of chronic hypoxia on optical density of μ-opioid receptors in fetal rat brain
Kraczkowski, Janusz Jacek
Karwasik-Kajszczarek, Katarzyna
Robak, Jacek Marcin
GINEKOLOGIA POLSKA, 2014, 85 (10) : 730 - 737
[36] The therapeutic effect of controlled reoxygenation on chronic hypoxia-associated brain injury
Tu, Zhenzhen
Tan, Xingqin
Li, Shangyingying
Cui, Jie
Tu, Shengfen
Jiang, Li
BIOLOGY OPEN, 2019, 8 (12):
[37] Effect of hypoxia-inducible factor 1-alpha on hypoxia/reoxygenation-induced apoptosis in primary neonatal rat cardiomyocytes
Wang, Xiaoou
Ma, Shuai
Qi, Guoxian
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2012, 417 (04) : 1227 - 1234
[38] The Anti-inflammatory Effect of Baicalin on Hypoxia/Reoxygenation and TNF-α Induced Injury in Cultural Rat Cardiomyocytes
Lin, Lin
Wu, Xiao-dong
Davey, Andrew K.
Wang, Jiping
PHYTOTHERAPY RESEARCH, 2010, 24 (03) : 429 - 437
[39] The protective effect of Hif3a RNA interference and HIF-prolyl hydroxylase inhibition on cardiomyocytes under anoxia-reoxygenation
Drevytska, T.
Gonchar, E.
Okhai, I.
Lynnyk, O.
Mankovska, I.
Klionsky, D.
Dosenko, V.
LIFE SCIENCES, 2018, 202 : 131 - 139
[40] Hypoxia-reoxygenation-induced apoptosis in cultured neonatal rat cardiomyocytes and the protective effect of prostaglandin E1
Ma, XQ
Fu, RF
Feng, GQ
Wang, ZJ
Ma, SG
Weng, SA
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY, 2005, 32 (12): : 1124 - 1130

← 1 2 3 4 5 →