Species diversity of Trichoderma in Poland

被引：72

作者：

Blaszczyk, Lidia ^{[1
]}

Popiel, Delfina ^{[1
]}

Chelkowski, Jerzy ^{[1
]}

Koczyk, Grzegorz ^{[1
]}

Samuels, Gary J. ^{[2
]}

Sobieralski, Krzysztof ^{[3
]}

Siwulski, Marek ^{[3
]}

机构：

[1] Polish Acad Sci, Inst Plant Genet, PL-60479 Poznan, Poland

[2] USDA, Systemat Mycol & Microbiol Lab, Agr Res Serv, Beltsville, MD 20705 USA

[3] Univ Life Sci, Dept Vegetable Crops, PL-60594 Poznan, Poland

来源：

JOURNAL OF APPLIED GENETICS | 2011年 / 52卷 / 02期

关键词：

Hypocreales; Molecular identification; ITS1; ITS2; rRNA; tef1; Phylogenetic analysis; Biogeography; CYTOTOXIC MODIFIED DIPEPTIDES; MULTIPLE SEQUENCE ALIGNMENT; AGARICUS-BISPORUS; GENETIC DIVERSITY; BIODIVERSITY; HYPOCREA; FUNGAL; LONGIBRACHIATUM; HYPOCREA/TRICHODERMA; BREVICOMPACTUM;

D O I：

10.1007/s13353-011-0039-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present study, we reinvestigate the diversity of Trichoderma in Poland utilizing a combination of morphological and molecular/phylogenetic methods. A total of 170 isolates were collected from six different substrata at 49 sites in Poland. These were divided among 14 taxa as follows: 110 of 170 Trichoderma isolates were identified to the species level by the analysis of their ITS1, ITS2 rDNA sequences as: T. harzianum (43 isolates), T. aggressivum (35), T. citrinoviride (11), T. hamatum (9), T. virens (6), T. longibrachiatum (4), T. polysporum (1), and T. tomentosum (1); 60 isolates belonging to the Viride clade were identified based on a fragment of the translation-elongation factor 1-alpha (tef1) gene as: T. atroviride (20 isolates), T. gamsii (2), T. koningii (17), T. viridescens (13), T. viride (7), and T. koningiopsis (1). Identifications were made using the BLAST interface in TrichOKEY and TrichoBLAST (http://www.isth.info). The most diverse substrata were soil (nine species per 22 isolates) and decaying wood (nine species per 75 isolates). The most abundant species (25%) isolated from all substrata was T. harzianum.

引用

页码：233 / 243

页数：11

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