A New Real-Time Reverse Transcription-PCR Assay for Detection of Human Enterovirus 68 in Respiratory Samples

被引：21

作者：

Piralla, Antonio ^{[1
]}

Girello, Alessia ^{[1
]}

Premoli, Marta ^{[1
]}

Baldanti, Fausto ^{[1
,2
]}

机构：

[1] Fdn IRCCS Policlin San Matteo, Mol Virol Unit, Microbiol & Virol Dept, Pavia, Italy

[2] Univ Pavia, Microbiol Sect, Dept Clin Surg Diagnost & Pediat Sci, I-27100 Pavia, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 05期

关键词：

ILLNESS;

D O I：

10.1128/JCM.03691-14

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.

引用

页码：1725 / 1726

页数：2

共 8 条

[1] Validation of Laboratory-Developed Molecular Assays for Infectious Diseases [J].

Burd, Eileen M. .

CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (03) :550-+

[2]

Centers for Disease Control and Prevention, 2014, Enterovirus D68 in the United States, 2014

[3]

Imamura Tadatsugu, 2011, Morbidity and Mortality Weekly Report, V60, P1301

[4] Continued seasonal circulation of enterovirus D68 in the Netherlands, 2011-2014 [J].

Meijer, A. ;

Benschop, K. S. ;

Donker, G. A. ;

van der Avoort, H. G. .

EUROSURVEILLANCE, 2014, 19 (42) :2-7

[5]

Midgley Claire M, 2014, MMWR Morb Mortal Wkly Rep, V63, P798

[6] Enterovirus 68 is associated with respiratory illness and shares biological features with both the enteroviruses and the rhinoviruses [J].

Oberste, MS ;

Maher, K ;

Schnurr, D ;

Flemister, MR ;

Lovchik, JC ;

Peters, H ;

Sessions, W ;

Kirk, C ;

Chatterjee, N ;

Fuller, S ;

Hanauer, JM ;

Pallansch, MA .

JOURNAL OF GENERAL VIROLOGY, 2004, 85 :2577-2584

[7] Phylogenetic Characterization of Enterovirus 68 Strains in Patients With Respiratory Syndromes in Italy [J].

Piralla, Antonio ;

Girello, Alessia ;

Grignani, Michela ;

Gozalo-Margueello, Monica ;

Marchi, Antonietta ;

Marseglia, Gianluigi ;

Baldanti, Fausto .

JOURNAL OF MEDICAL VIROLOGY, 2014, 86 (09) :1590-1593

[8] Human rhinovirus and human respiratory enterovirus (EV68 and EV104) in hospitalized patients in Italy, 2008-2009 [J].

Piralla, Antonio ;

Lilleri, Daniele ;

Sarasini, Antonella ;

Marchi, Antonietta ;

Zecca, Marco ;

Stronati, Mauro ;

Baldanti, Fausto ;

Gerna, Giuseppe .

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 73 (02) :162-167

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