A Comparison of Recombinant Receptor-Reporter Gene Bioassays and a Total Estrogen Enzyme Linked Immunosorbent Assay for the Rapid Screening of Estrogenic Activity in Natural and Waste Waters

被引：10

作者：

Allinson, Mayumi ^{[1
,2
]}

Shiraishi, Fujio ^{[3
]}

Allinson, Graeme ^{[1
,2
]}

机构：

[1] Dept Primary Ind, Future Farming Syst Res Div, Queenscliff, Vic 3225, Australia

[2] Univ Melbourne, CAPIM, Bio Inst 21, Parkville, Vic 3052, Australia

[3] Natl Inst Environm Studies, Environm Qual Measurement Sect, Res Ctr Environm Risk, Tsukuba, Ibaraki 3058506, Japan

来源：

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY | 2011年 / 86卷 / 05期

关键词：

Estrogenic activity; Recombinant receptor-reporter gene bioassay; Total estrogens ELISA; TREATMENT PLANTS; AUSTRALIA; EFFLUENT; VICTORIA;

D O I：

10.1007/s00128-011-0251-x

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

In this study, we compared the performance as screening tools of two yeast-based recombinant receptor-reporter gene bioassays with a commercial ELISA kit for measurement of total estrogens. For WWTP effluents there was a very good correlation between the measured total estrogen concentrations (ELISA) and estrogenic activity by the hER alpha bioassay (r(2) = 0.93), but not for the medER alpha bioassay (r(2) = 0.50). For freshwater samples, the correlations between bioassay response and ELISA ES measurments were very good (r(2) > 0.95). There was no correlation between bioassay response and ELISA ES measurments for estuarine samples.

引用

页码：461 / 464

页数：4

共 12 条

[1] Androgenic activity of effluent from forty-five municipal waste water treatment plants in Victoria, Australia [J].