Establishment and characterization of an embryonic cell line from Gampsocleis gratiosa (Orthoptera: Tettigoniidae)

被引：12

作者：

Zhang, Xin ^{[1
]}

Feng, Ying ^{[1
]}

Ding, Wei-Feng ^{[1
]}

Chen, Xiao-Ming ^{[1
]}

Wang, Cheng-Ye ^{[1
]}

Ma, Tao ^{[1
]}

机构：

[1] Chinese Acad Forestry, Res Inst Resource Insects, Kunming 650224, Yunnan, Peoples R China

来源：

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL | 2011年 / 47卷 / 04期

关键词：

Gampsocleis gratiosa; Orthoptera; Embryonic cell line; Characterization; NUCLEAR POLYHEDROSIS-VIRUS; RECOMBINANT PROTEINS; VIRAL SUSCEPTIBILITY; SPODOPTERA-EXIGUA; LEPIDOPTERA; REPLICATION; EXPRESSION; NOCTUIDAE; CULTURE;

D O I：

10.1007/s11626-011-9397-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The first continuous cell line from the embryo of Gampsocleis gratiosa (Orthoptera: Tettigoniidae), designated as RIRI-GG1, was established. This cell line was serially subcultured in modified Grace medium. The cells were grown adherent to a culture flask and had spindle-like and polygonal shapes. The chromosome number ranged from 26 to 79 at the 50th passage, and 68% of cells had a diploid chromosome number. The growth rate was determined at the 53rd passage, and the population doubling time was calculated to be 122.1 h. The rDNA internal transcribed spacer and the mitochondrial cytochrome c oxidase subunit I gene sequence analysis indicated that the RIRI-GG1 cell line was derived from G. gratiosa. This cell line had no apparent susceptibility to Autographa californica nucleopolyhedrovirus and Bombyx mori nucleopolyhedrovirus.

引用

页码：327 / 332

页数：6

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