Production of recombinant hirudin in Hansenula polymorpha: variation of gene expression level depends on methanol oxidase and fermentation strategies

被引：0

作者：

Kim, CH ^{[1
]}

Seo, HW ^{[1
]}

Kim, HY ^{[1
]}

Sohn, JH ^{[1
]}

Choi, ES ^{[1
]}

Rhee, SK ^{[1
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, Appl Microbiol Res Div, Taejon 305600, South Korea

来源：

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY | 1998年 / 21卷 / 1-2期

关键词：

hirudin; Hansenula polymorpha; methylotrophic yeast; methanol oxidase; autonomously replicating sequence;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Hansenula polymorpha strains were developed and compared for their level of expression of the anticoagulant hirudin, H. polymorpha DL1-57 harboring an autonomously replicating sequence, HARS36, efficiently expressed the gene for recombinant hirudin, The effect of methanol oxidase (MOX) on the expression of the hirudin gene in H. polymorpha DL1-57 was studied, and the fermentation strategies coupled with the MOX activity and an antioxidant, tocopherol, were also examined.

引用

页码：1 / 5

页数：5

共 13 条

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