Background: Enterotoxigenic Escherichia coli (ETEC) infections result in large economic losses in the swine industry worldwide. ETEC infections cause pro-inflammatory responses in intestinal epithelial cells and subsequent diarrhea in pigs, leading to reduced growth rate and mortality. Administration of probiotics as feed additives displayed health benefits against intestinal infections. Saccharomyces cerevisiae (Sc) is non-commensal and non-pathogenic yeast used as probiotic in gastrointestinal diseases. However, the immuno-modulatory effects of Sc in differentiated porcine intestinal epithelial cells exposed to ETEC were not investigated. Methodology/Principal Findings: We reported that the yeast Sc (strain CNCM I-3856) modulates transcript and protein expressions involved in inflammation, recruitment and activation of immune cells in differentiated porcine intestinal epithelial IPEC-1 cells. We demonstrated that viable Sc inhibits the ETEC-induced expression of pro-inflammatory transcripts (IL-6, IL-8, CCL20, CXCL2, CXCL10) and proteins (IL-6, IL-8). This inhibition was associated to a decrease of ERK1/2 and p38 MAPK phosphorylation, an agglutination of ETEC by Sc and an increase of the anti-inflammatory PPAR-c nuclear receptor mRNA level. In addition, Sc up-regulates the mRNA levels of both IL-12p35 and CCL25. However, measurement of transepithelial electrical resistance displayed that Sc failed to maintain the barrier integrity in monolayer exposed to ETEC suggesting that Sc does not inhibit ETEC enterotoxin activity. Conclusions: Sc (strain CNCM I-3856) displays multiple immuno-modulatory effects at the molecular level in IPEC-1 cells suggesting that Sc may influence intestinal inflammatory reaction.
机构:
TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, D-44139 Dortmund, GermanyInst Mol Pathogenesis, Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, D-07743 Jena, Germany
Foellmann, Wolfram
;
Birkner, Sascha
论文数: 0引用数: 0
h-index: 0
机构:
TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, D-44139 Dortmund, GermanyInst Mol Pathogenesis, Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, D-07743 Jena, Germany
机构:
Barts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Bustin, Stephen A.
;
Benes, Vladimir
论文数: 0引用数: 0
h-index: 0
机构:
EMBL Heidelberg, Genom Core Facil, Heidelberg, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Benes, Vladimir
;
Garson, Jeremy A.
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Dept Infect, Ctr Virol, London, England
UCL Hosp, NHS Fdn Trust, Dept Virol, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Garson, Jeremy A.
;
Hellemans, Jan
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Hellemans, Jan
;
Huggett, Jim
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Ctr Infect Dis, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Huggett, Jim
;
Kubista, Mikael
论文数: 0引用数: 0
h-index: 0
机构:
TATAA Bioctr, Gothenburg, Sweden
Inst Biotechnol AS CR, Prague, Czech RepublicBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Kubista, Mikael
;
Mueller, Reinhold
论文数: 0引用数: 0
h-index: 0
机构:
Sequenom, San Diego, CA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Mueller, Reinhold
;
Nolan, Tania
论文数: 0引用数: 0
h-index: 0
机构:
Sigma Aldrich, Haverhill, MA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Nolan, Tania
;
Pfaffl, Michael W.
论文数: 0引用数: 0
h-index: 0
机构:
Tech Univ Munich, D-8050 Freising Weihenstephan, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Pfaffl, Michael W.
;
Shipley, Gregory L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas Houston, Hlth Sci Ctr, Dept Integrat Biol & Pharmacol, Quantitat Genom Core Lab, Houston, TX USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Shipley, Gregory L.
;
Vandesompele, Jo
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Vandesompele, Jo
;
Wittwer, Carl T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Utah, Dept Pathol, Salt Lake City, UT USA
ARUP Inst Clin & Expt Pathol, Salt Lake City, UT USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Castagliuolo, I
;
Riegler, MF
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Riegler, MF
;
Valenick, L
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Valenick, L
;
LaMont, JT
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
LaMont, JT
;
Pothoulakis, C
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Casella, Carolyn R.
;
Eaves, Chelsea A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Eaves, Chelsea A.
;
Matsuzawa, Atsushi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Pharmaceut Sci, Lab Cell Signaling, Tokyo 1130033, JapanUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Matsuzawa, Atsushi
;
Ichijo, Hidenori
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Pharmaceut Sci, Lab Cell Signaling, Tokyo 1130033, JapanUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Ichijo, Hidenori
;
Mitchell, Thomas C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
机构:
TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, D-44139 Dortmund, GermanyInst Mol Pathogenesis, Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, D-07743 Jena, Germany
Foellmann, Wolfram
;
Birkner, Sascha
论文数: 0引用数: 0
h-index: 0
机构:
TU Dortmund, Leibniz Res Ctr Working Environm & Human Factors, D-44139 Dortmund, GermanyInst Mol Pathogenesis, Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, D-07743 Jena, Germany
机构:
Barts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Bustin, Stephen A.
;
Benes, Vladimir
论文数: 0引用数: 0
h-index: 0
机构:
EMBL Heidelberg, Genom Core Facil, Heidelberg, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Benes, Vladimir
;
Garson, Jeremy A.
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Dept Infect, Ctr Virol, London, England
UCL Hosp, NHS Fdn Trust, Dept Virol, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Garson, Jeremy A.
;
Hellemans, Jan
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Hellemans, Jan
;
Huggett, Jim
论文数: 0引用数: 0
h-index: 0
机构:
UCL, Ctr Infect Dis, London, EnglandBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Huggett, Jim
;
Kubista, Mikael
论文数: 0引用数: 0
h-index: 0
机构:
TATAA Bioctr, Gothenburg, Sweden
Inst Biotechnol AS CR, Prague, Czech RepublicBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Kubista, Mikael
;
Mueller, Reinhold
论文数: 0引用数: 0
h-index: 0
机构:
Sequenom, San Diego, CA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Mueller, Reinhold
;
Nolan, Tania
论文数: 0引用数: 0
h-index: 0
机构:
Sigma Aldrich, Haverhill, MA USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Nolan, Tania
;
Pfaffl, Michael W.
论文数: 0引用数: 0
h-index: 0
机构:
Tech Univ Munich, D-8050 Freising Weihenstephan, GermanyBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Pfaffl, Michael W.
;
Shipley, Gregory L.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Texas Houston, Hlth Sci Ctr, Dept Integrat Biol & Pharmacol, Quantitat Genom Core Lab, Houston, TX USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Shipley, Gregory L.
;
Vandesompele, Jo
论文数: 0引用数: 0
h-index: 0
机构:
Ghent Univ Hosp, Ctr Med Genet, B-9000 Ghent, BelgiumBarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
Vandesompele, Jo
;
Wittwer, Carl T.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Utah, Dept Pathol, Salt Lake City, UT USA
ARUP Inst Clin & Expt Pathol, Salt Lake City, UT USABarts & London Queen Marys Sch Med & Dent, Inst Cell & Mol Sci, Ctr Acad Surg, London, England
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Castagliuolo, I
;
Riegler, MF
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Riegler, MF
;
Valenick, L
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
Valenick, L
;
LaMont, JT
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
LaMont, JT
;
Pothoulakis, C
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USAHarvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Div Gastroenterol, Boston, MA USA
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Casella, Carolyn R.
;
Eaves, Chelsea A.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Eaves, Chelsea A.
;
Matsuzawa, Atsushi
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Pharmaceut Sci, Lab Cell Signaling, Tokyo 1130033, JapanUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Matsuzawa, Atsushi
;
Ichijo, Hidenori
论文数: 0引用数: 0
h-index: 0
机构:
Univ Tokyo, Grad Sch Pharmaceut Sci, Lab Cell Signaling, Tokyo 1130033, JapanUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA
Ichijo, Hidenori
;
Mitchell, Thomas C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USAUniv Louisville, Inst Cellular Therapeut, Sch Med, Donald Baxter Res Bldg,570 S Preston St,Rm 410, Louisville, KY 40202 USA