Saccharomyces cerevisiae Modulates Immune Gene Expressions and Inhibits ETEC-Mediated ERK1/2 and p38 Signaling Pathways in Intestinal Epithelial Cells

被引:113
作者
Zanello, Galliano [1 ,2 ]
Berri, Mustapha [2 ]
Dupont, Joelle [3 ]
Sizaret, Pierre-Yves [4 ]
D'Inca, Romain [1 ]
Salmon, Henri [2 ]
Meurens, Francois [2 ]
机构
[1] Soc Ind Lesaffre, Marcq En Baroeul, France
[2] INRA, UR1282, Tours, France
[3] INRA, UMR85, Tours, France
[4] Univ Tours, Dept Microscopies, Plate Forme RIO Microscopie Elect, Tours, France
关键词
ENTEROTOXIGENIC ESCHERICHIA-COLI; INFECTED T84 CELLS; DIFFICILE TOXIN-A; NF-KAPPA-B; IN-VITRO; ANTIMICROBIAL RESISTANCE; TRANSCRIPTION FACTORS; VIRULENCE FACTORS; COLONIC-MUCOSA; BOULARDII;
D O I
10.1371/journal.pone.0018573
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Enterotoxigenic Escherichia coli (ETEC) infections result in large economic losses in the swine industry worldwide. ETEC infections cause pro-inflammatory responses in intestinal epithelial cells and subsequent diarrhea in pigs, leading to reduced growth rate and mortality. Administration of probiotics as feed additives displayed health benefits against intestinal infections. Saccharomyces cerevisiae (Sc) is non-commensal and non-pathogenic yeast used as probiotic in gastrointestinal diseases. However, the immuno-modulatory effects of Sc in differentiated porcine intestinal epithelial cells exposed to ETEC were not investigated. Methodology/Principal Findings: We reported that the yeast Sc (strain CNCM I-3856) modulates transcript and protein expressions involved in inflammation, recruitment and activation of immune cells in differentiated porcine intestinal epithelial IPEC-1 cells. We demonstrated that viable Sc inhibits the ETEC-induced expression of pro-inflammatory transcripts (IL-6, IL-8, CCL20, CXCL2, CXCL10) and proteins (IL-6, IL-8). This inhibition was associated to a decrease of ERK1/2 and p38 MAPK phosphorylation, an agglutination of ETEC by Sc and an increase of the anti-inflammatory PPAR-c nuclear receptor mRNA level. In addition, Sc up-regulates the mRNA levels of both IL-12p35 and CCL25. However, measurement of transepithelial electrical resistance displayed that Sc failed to maintain the barrier integrity in monolayer exposed to ETEC suggesting that Sc does not inhibit ETEC enterotoxin activity. Conclusions: Sc (strain CNCM I-3856) displays multiple immuno-modulatory effects at the molecular level in IPEC-1 cells suggesting that Sc may influence intestinal inflammatory reaction.
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页数:13
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