Reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) as a user-friendly system to detect SARS-CoV-2 infection: a multicentric study

被引：0

作者：

Stracquadanio, Stefano ^{[1
]}

Di Gaudio, Francesca ^{[2
]}

Giunta, Elena ^{[3
]}

Falliti, Giuseppe ^{[3
]}

Caruso, Daniele ^{[3
]}

Pinzone, Carmelo ^{[3
]}

Noto, Patrizia ^{[3
]}

Di Naso, Concettina ^{[4
]}

Garozzo, Sebastiano Fabio ^{[4
]}

Amodeo, Andrea ^{[4
]}

Cina, Diana

Cardillo, Maria Concetta ^{[5
]}

Zappala, Simona ^{[5
]}

Consoli, Alessandro ^{[5
]}

Stefani, Stefania ^{[1
]}

机构：

[1] Univ Catania, Dept Biomed & Biotechnol Sci, Med Mol Microbiol & Antibiot Resistance Lab, Catania, Italy

[2] Univ Palermo, Dept Promoting Hlth Maternal Infant Excellence &, Palermo, Italy

[3] Papardo Hosp Contrada Papardo, Dept Serv, Complex Operat Unit Clin Pathol, Messina, Italy

[4] PO Garibaldi Ctr, Complex Operat Unit Clin Pathol & Clin Mol Biol, Catania, Italy

[5] Complex Operat Unit Clin Pathol AEO Cannizzaro, Catania, Italy

来源：

NEW MICROBIOLOGICA | 2021年 / 44卷 / 03期

关键词：

RT-LAMP; COVID-19; diagnostic power; molecular tests; multicentric study;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Although reverse transcriptase quantitative PCR remains the gold standard to perform viral detection, reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) is already used to perform diagnosis of various infections. This work reports the results of a multicentric study performed in Sicily to evaluate the diagnostic power of an RT-LAMP kit for the diagnosis of SARS-CoV-2 infection on a total of 551 samples collected in January and February 2021, revealing sensitivity, specificity, accuracy, positive and negative predictive values >= 95%. Our results suggest the potential employment of this kit as a screening test to be used where fast and reliable results are demanded without the need for expensive instruments and highly-skilled personnel.

引用

页码：181 / 183

页数：3

共 7 条

[1] Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis [J].