Inhibition of prostate cancer cell growth in vivo with short hairpin RNA targeting SATB1

被引：4

作者：

Wang, Qiang ^{[1
]}

Hu, Shi-Cheng ^{[2
]}

Yang, Chun-Sheng ^{[3
]}

Chen, Jia-Cun ^{[2
]}

Zheng, Jun-Nian ^{[4
]}

Sun, Xiao-Qing ^{[2
]}

Wang, Jun-Qi ^{[2
]}

机构：

[1] Nanjing Univ Chinese Med, Clin Med Coll 1, Nanjing 210046, Jiangsu, Peoples R China

[2] Xuzhou Med Coll, Affiliated Hosp, Dept Urol, 99 Huaihai Rd West, Xuzhou 221002, Jiangsu, Peoples R China

[3] Xuzhou Med Univ, Peoples Hosp Huaian 2, Affiliated Huaian Hosp, Dept Dermatol, Huaian 223002, Peoples R China

[4] Xuzhou Med Coll, Affiliated Hosp, Ctr Clin Oncol, Xuzhou 221002, Jiangsu, Peoples R China

来源：

ONCOLOGY LETTERS | 2017年 / 14卷 / 06期

关键词：

special AT-rich sequence binding protein 1; prostate cancer; short hairpin RNA; apoptosis; GENE-THERAPY; INTERFERENCE; METASTASIS;

D O I：

10.3892/ol.2017.7006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Despite previous advances, the treatment options for prostate cancer remain limited. For the purposes of gene knockdown, the utility of RNA interference has been demonstrated and is considered to have therapeutic potential. In the present study, a short hairpin RNA (shRNA) was used to assess the effect of special AT-rich sequence binding protein (SATB1) downregulation on the growth and metastatic potential of prostate cancer in xenograft nude mice. A plasmid carrying shRNA targeting SATB1, pSilencer-SATB1-shRNA, was successfully engineered. Using this plasmid, significant downregulation of SATB1 mRNA and protein expression in the DU145 prostate cancer cells was observed. pSilencer-SATB1-shRNA was demonstrated to be markedly efficacious against prostate cancer xenografts in nude mice. These results may lead to a novel method of improving gene therapy efficacy against prostate cancer via regulating the function of SATB1.

引用

页码：6592 / 6596

页数：5

共 17 条

[1] RNA interference: Biology, mechanism, and applications [J].

Agrawal, N ;

Dasaradhi, PVN ;

Mohmmed, A ;

Malhotra, P ;

Bhatnagar, RK ;

Mukherjee, SK .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2003, 67 (04) :657-+

[2] Prodrug cancer gene therapy [J].

Altaner, Cestmir .

CANCER LETTERS, 2008, 270 (02) :191-201

[3] RNA interference: A futuristic tool and its therapeutic applications [J].

Ambesajir, Anghesom ;

Kaushik, Atul ;

Kaushik, Jeevan J. ;

Petros, Sham T. .

SAUDI JOURNAL OF BIOLOGICAL SCIENCES, 2012, 19 (04) :395-403

[4] RNA interference therapeutics for cancer: Challenges and opportunities (Review) [J].

Bora, Roop Singh ;

Gupta, Dikshi ;

Mukkur, Trilochan Kanwaljit Singh ;

Saini, Kulvinder Singh .

MOLECULAR MEDICINE REPORTS, 2012, 6 (01) :9-15

[5]

Gridley Daila S, 2004, Discov Med, V4, P408

[6] SATB1 reprogrammes gene expression to promote breast tumour growth and metastasis [J].

Han, Hye-Jung ;

Russo, Jose ;

Kohwi, Yoshinori ;

Kohwi-Shigematsu, Terumi .

NATURE, 2008, 452 (7184) :187-193

[7] Genome organizing function of SATB1 in tumor progression [J].

Kohwi-Shigematsu, Terumi ;

Poterlowicz, Krzysztof ;

Ordinario, Ellen ;

Han, Hye-Jung ;

Botchkarev, Vladimir A. ;

Kohwi, Yoshinori .

SEMINARS IN CANCER BIOLOGY, 2013, 23 (02) :72-79

[8] The nuclear RNase III Drosha initiates microRNA processing [J].

Lee, Y ;

Ahn, C ;

Han, JJ ;

Choi, H ;

Kim, J ;

Yim, J ;

Lee, J ;

Provost, P ;

Rådmark, O ;

Kim, S ;

Kim, VN .

NATURE, 2003, 425 (6956) :415-419

[9] RNA Interference and its Role in Cancer Therapy [J].

Mansoori, Behzad ;

Shotorbani, Siamak Sandoghchian ;

Baradaran, Behzad .

ADVANCED PHARMACEUTICAL BULLETIN, 2014, 4 (04) :313-321

[10] Oncolytic virus carrying shRNA targeting SATB1 inhibits prostate cancer growth and metastasis [J].

Mao, Li-jun ;

Zhang, Jie ;

Liu, Ning ;

Fan, Li ;

Yang, Dong-rong ;

Xue, Bo-xin ;

Shan, Yu-xi ;

Zheng, Jun-nian .

TUMOR BIOLOGY, 2015, 36 (11) :9073-9081

← 1 2 →