Chromatin "Prepattern" and Histone Modifiers in a Fate Choice for Liver and Pancreas

被引：158

作者：

Xu, Cheng-Ran ^{[1
]}

Cole, Philip A. ^{[2
]}

Meyers, David J. ^{[2
]}

Kormish, Jay ^{[1
]}

Dent, Sharon ^{[3
]}

Zaret, Kenneth S. ^{[1
]}

机构：

[1] Univ Penn, Sch Med, Dept Cell & Dev Biol, Inst Regenerat Med,Epigenet Program, Philadelphia, PA 19104 USA

[2] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Mol Carcinogenesis, Smithville, TX 78957 USA

来源：

SCIENCE | 2011年 / 332卷 / 6032期

关键词：

TRANSCRIPTION FACTORS; STEM-CELLS; IN-VITRO; GENE; EXPRESSION; ENDODERM; PROMOTER; DISTINCT; ACETYLTRANSFERASE; DIFFERENTIATION;

D O I：

10.1126/science.1202845

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcriptionally silent genes can be marked by histone modifications and regulatory proteins that indicate the genes' potential to be activated. Such marks have been identified in pluripotent cells, but it is unknown how such marks occur in descendant, multipotent embryonic cells that have restricted cell fate choices. We isolated mouse embryonic endoderm cells and assessed histone modifications at regulatory elements of silent genes that are activated upon liver or pancreas fate choices. We found that the liver and pancreas elements have distinct chromatin patterns. Furthermore, the histone acetyltransferase P300, recruited via bone morphogenetic protein signaling, and the histone methyltransferase Ezh2 have modulatory roles in the fate choice. These studies reveal a functional "prepattern" of chromatin states within multipotent progenitors and potential targets to modulate cell fate induction.

引用

页码：963 / 966

页数：4

共 36 条

[1] Chromatin signatures of pluripotent cell lines [J].

Azuara, V ;

Perry, P ;

Sauer, S ;

Spivakov, M ;

Jorgensen, HF ;

John, RM ;

Gouti, M ;

Casanova, M ;

Warnes, G ;

Merkenschlager, M ;

Fisher, AG .

NATURE CELL BIOLOGY, 2006, 8 (05) :532-U189

[2] A bivalent chromatin structure marks key developmental genes in embryonic stem cells [J].

Bernstein, BE ;

Mikkelsen, TS ;

Xie, XH ;

Kamal, M ;

Huebert, DJ ;

Cuff, J ;

Fry, B ;

Meissner, A ;

Wernig, M ;

Plath, K ;

Jaenisch, R ;

Wagschal, A ;

Feil, R ;

Schreiber, SL ;

Lander, ES .

CELL, 2006, 125 (02) :315-326

[3] Hex homeobox gene-dependent tissue positioning is required for organogenesis of the ventral pancreas [J].

Bort, R ;

Martinez-Barbera, JP ;

Beddington, RSP ;

Zaret, KS .

DEVELOPMENT, 2004, 131 (04) :797-806

[4] Virtual Ligand Screening of the p300/CBP Histone Acetyltransferase: Identification of a Selective Small Molecule Inhibitor [J].

Bowers, Erin M. ;

Yan, Gai ;

Mukherjee, Chandrani ;

Orry, Andrew ;

Wang, Ling ;

Holbert, Marc A. ;

Crump, Nicholas T. ;

Hazzalin, Catherine A. ;

Liszczak, Glen ;

Yuan, Hua ;

Larocca, Cecilia ;

Saldanha, S. Adrian ;

Abagyan, Ruben ;

Sun, Yan ;

Meyers, David J. ;

Marmorstein, Ronen ;

Mahadevan, Louis C. ;

Alani, Rhoda M. ;

Cole, Philip A. .

CHEMISTRY & BIOLOGY, 2010, 17 (05) :471-482

[5] Complementation rescue of Pdx1 null phenotype demonstrates distinct roles of proximal and distal cis-regulatory sequences in pancreatic and duodenal expression [J].

Boyer, Daniel F. ;

Fujitani, Yoshio ;

Gannon, Maureen ;

Powers, Alvin C. ;

Stein, Roland W. ;

Wright, Christopher V. E. .

DEVELOPMENTAL BIOLOGY, 2006, 298 (02) :616-631

[6] Role of histone H3 lysine 27 methylation in polycomb-group silencing [J].

Cao, R ;

Wang, LJ ;

Wang, HB ;

Xia, L ;

Erdjument-Bromage, H ;

Tempst, P ;

Jones, RS ;

Zhang, Y .

SCIENCE, 2002, 298 (5595) :1039-1043

[7] Differential requirements for Smad4 in TGFβ-dependent patterning of the early mouse embryo [J].

Chu, GC ;

Dunn, NR ;

Anderson, DC ;

Oxburgh, L ;

Robertson, EJ .

DEVELOPMENT, 2004, 131 (15) :3501-3512

[8] MULTIPLE HEPATOCYTE-ENRICHED NUCLEAR FACTORS FUNCTION IN THE REGULATION OF TRANSTHYRETIN AND ALPHA-1-ANTITRYPSIN GENES [J].

COSTA, RH ;

GRAYSON, DR ;

DARNELL, JE .

MOLECULAR AND CELLULAR BIOLOGY, 1989, 9 (04) :1415-1425

[9] A rapid micro chromatin immunoprecipitation assay (μChIP) [J].

Dahl, John Arne ;

Collas, Philippe .

NATURE PROTOCOLS, 2008, 3 (06) :1032-1045

[10] The Smad4 activation domain (SAD) is a proline-rich, p300-dependent transcriptional activation domain [J].

de Caestecker, MP ;

Yahata, T ;

Wang, D ;

Parks, WT ;

Huang, SX ;

Hill, CS ;

Shioda, T ;

Roberts, AB ;

Lechleider, RJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (03) :2115-2122

← 1 2 3 4 →