Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning

被引：68
|
作者：
Cheng, Jun [1 ,2 ,3 ]
Tiyaboonchai, Amita [1 ,2 ]
Yamashita, Yukiko M. [1 ,2 ,4 ]
Hunt, Alan J. [3 ]
机构：
[1] Ctr Stem Cell Biol, Inst Life Sci, Ann Arbor, MI 48109 USA
[2] Sch Med, Dept Cell & Dev Biol, Inst Life Sci, Ann Arbor, MI 48109 USA
[3] Ctr Ultrafast Opt Sci, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[4] Univ Michigan, Cellular & Mol Biol Program, Ann Arbor, MI 48109 USA
来源：
DEVELOPMENT | 2011年 / 138卷 / 05期
关键词：
Stem cells; Asymmetric division; Spindle repositioning; Drosophila; SOMATIC SUPPORT CELLS; KINESIN-LIKE PROTEIN; SELF-RENEWAL; CENTROSOMIN PROTEIN; GERM-CELLS; MOESIN; NICHE; ACTIN; MORPHOGENESIS; MELANOGASTER;
D O I：
10.1242/dev.057901
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Many stem cells divide asymmetrically to balance self-renewal and differentiation. In Drosophila testes, two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs), cohere and regulate one another. Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin. Anaphase spindle repositioning is required to achieve high-fidelity asymmetric divisions in CySCs, thus maintaining both GSC and CySC numbers. We propose that dynamic spindle repositioning allows CySCs to divide asymmetrically while accommodating the structure of the GSCs they encapsulate.
引用
收藏
页码：831 / 837
页数：7
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