Vitexin Glucolone Reinforces Radiosensitivity of Non-Small Cell Lung Cancer via Transforming Growth Factor Kinase 1/Adenylate Activated Protein Kinase Signaling Pathway

Purpose: To discuss effects of vitexin glucolone (VG) to radiosensitivity of NSCLC (Non-small cell lung cancer) cell lines (A549 and H1299). Methods: Treating A549 and H1299 cells by VG with or without X-radiation. Cell viability was calculated by CCK8. Apoptosis rate was measured by flow cytometry and Western blot to expressions of protein. Subsequently, cells were transferred with TAK1 siRNA, cell viability and apoptosis were measured. Results: VG decreased the viability of Huh7 cells and inhibited effects of VG was more strengthen than radiotherapy in concentrations of 20 mu mol/L and 40 mu mol/L. Meanwhile, VG sensitized HCC exposed to radiation therapy to apoptosis as demonstrated by increased Bax/Bcl-2 ratio. In addition, VG enhanced the promotive effects of X-radiation on the expressions of TAK1, AMPKa1 and PPARy. Furthermore, silence the expression of TAK1 partly reversed the effects of VG on HCC and radiosensitivity of NSCLC. Conclusion: VG enhances radiosensitivity of NSCLC via TAK1/AMPK pathway.