Quality control in combinatorial chemistry: Determinations of amounts and comparison of the "purity" of LC-MS-purified samples by NMR, LC-UV and CLND

被引：27

作者：

Letot, E ^{[1
]}

Koch, G ^{[1
]}

Falchetto, R ^{[1
]}

Bovermann, G ^{[1
]}

Oberer, L ^{[1
]}

Roth, HJ ^{[1
]}

机构：

[1] Novartis Inst BioMed Res, CH-4002 Basel, Switzerland

来源：

JOURNAL OF COMBINATORIAL CHEMISTRY | 2005年 / 7卷 / 03期

关键词：

D O I：

10.1021/cc049850r

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The absolute purities of 20 purified samples from a combinatorial library have been determined by a new method that uses the DMSO sidebands [(1)J{C-13-H-1}] as an internal standard for quantification. The obtained absolute amounts are compared with the amounts of sample obtained by weighing, with the calculated weights obtained by chemiluminescent nitrogen detection (CLND) chromatography and with the relative purities obtained by LC-UV chromatography.

引用

页码：364 / 371

页数：8

共 3 条

[1] Versatile monitoring tools in parallel solid-phase synthesis
Felder, ER
Martina, K
Scarpella, S
Tatò, M
[J]. CHIMIA, 2003, 57 (05) : 229 - 236
[2] Simultaneous on-line characterization of small organic molecules derived from combinatorial libraries for identity, quantity, and purity by reversed-phase HPLC with chemiluminescent nitrogen, UV, and mass spectrometric detection
Taylor, EW
Qian, MG
Dollinger, GD
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (16) : 3339 - 3347
[3] Quality control in combinatorial chemistry: Determination of the quantity, purity, and quantitative purity of compounds in combinatorial libraries
Yan, B
Fang, LL
Irving, M
Zhang, S
Boldi, AM
Woolard, F
Johnson, CR
Kshirsagar, T
Figliozzi, GM
Krueger, CA
Collins, N
[J]. JOURNAL OF COMBINATORIAL CHEMISTRY, 2003, 5 (05): : 547 - 559

← 1 →