Expression level and activity profile of membrane bound guanylate cyclase type 2 in rod outer segments

被引:38
作者
Helten, Andreas [1 ]
Saeftel, Werner [1 ]
Koch, Karl-Wilhelm [1 ]
机构
[1] Carl Von Ossietzky Univ Oldenburg, Fac V, Inst Environm & Biol Sci, Biochem Grp, D-26111 Oldenburg, Germany
关键词
guanylate cyclase-activating protein; guanylate cyclase; light adaptation; neuronal Ca2+ sensors; phototransduction;
D O I
10.1111/j.1471-4159.2007.04923.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Rod and cone cells of the mammalian retina harbor two types of a membrane bound guanylate cyclase (GC), rod outer segment guanylate cyclase type 1 (ROS-GC1) and ROS-GC2. Both enzymes are regulated by small Ca2+-binding proteins named GC-activating proteins that operate as Ca2+ sensors and enable cyclases to respond to changes of intracellular Ca(2+)after illumination. We determined the expression level of ROS-GC2 in bovine ROS preparations and compared it with the level of ROS-GC1 in ROSs. The molar ratio of a ROS-GC2 dimer to rhodopsin was 1 : 13 200. The amount of ROS-GC1 was 25-fold higher than the amount of ROS-GC2. Heterologously expressed ROS-GC2 was differentially activated by GC-activating protein 1 and 2 at low free Ca2+ concentrations. Mutants of GC-activating protein 2 modulated ROS-GC2 in a manner different from their action on ROS-GC1 indicating that the Ca2+ sensitivity of the Ca2+ sensor is controlled by the mode of target-sensor interaction.
引用
收藏
页码:1439 / 1446
页数:8
相关论文
共 44 条
[21]   FUNCTIONAL COUPLING OF A CA2+/CALMODULIN-DEPENDENT NITRIC-OXIDE SYNTHASE AND A SOLUBLE GUANYLYL CYCLASE IN VERTEBRATE PHOTORECEPTOR CELLS [J].
KOCH, KW ;
LAMBRECHT, HG ;
HABERECHT, M ;
REDBURN, D ;
SCHMIDT, HHHW .
EMBO JOURNAL, 1994, 13 (14) :3312-3320
[22]   Mapping sites in guanylyl cyclase activating protein-1 required for regulation of photoreceptor membrane guanylyl cyclases [J].
Krylov, DM ;
Niemi, GA ;
Dizhoor, AM ;
Hurley, JB .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (16) :10833-10839
[23]   The kinase homology domain of retinal guanylyl cyclases 1 and 2 specifies the affinity and cooperativity of interaction with guanylyl cyclase activating protein-2 [J].
Laura, RP ;
Hurley, JB .
BIOCHEMISTRY, 1998, 37 (32) :11264-11271
[24]   ULTRASTRUCTURAL-LOCALIZATION OF RETINAL GUANYLATE-CYCLASE IN HUMAN AND MONKEY RETINAS [J].
LIU, XR ;
SENO, K ;
NISHIZAWA, Y ;
HAYASHI, F ;
YAMAZAKI, A ;
MATSUMOTO, H ;
WAKABAYASHI, T ;
USUKURA, J .
EXPERIMENTAL EYE RESEARCH, 1994, 59 (06) :761-768
[25]   CLONING AND EXPRESSION OF A 2ND PHOTORECEPTOR-SPECIFIC MEMBRANE RETINA GUANYLYL CYCLASE (RETGC), RETGC-2 [J].
LOWE, DG ;
DIZHOOR, AM ;
LIU, K ;
GU, QM ;
SPENCER, M ;
LAURA, R ;
LU, L ;
HURLEY, JB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) :5535-5539
[26]   STRUCTURAL AND BIOCHEMICAL IDENTITY OF RETINAL ROD OUTER SEGMENT MEMBRANE GUANYLATE-CYCLASE [J].
MARGULIS, A ;
GORACZNIAK, RM ;
DUDA, T ;
SHARMA, RK ;
SITARAMAYYA, A .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 194 (02) :855-861
[27]   Analysis of the functional coupling between calmodulin's calcium binding and peptide recognition properties [J].
Mirzoeva, S ;
Weigand, S ;
Lukas, TJ ;
Shuvalova, L ;
Anderson, WF ;
Watterson, DM .
BIOCHEMISTRY, 1999, 38 (13) :3936-3947
[28]  
Nakatani K, 2002, ADV EXP MED BIOL, V514, P1
[29]   Factors that affect regulation of cGMP synthesis in vertebrate photoreceptors and their genetic link to human retinal degeneration [J].
Olshevskaya, EV ;
Ermilov, AN ;
Dizhoor, AM .
MOLECULAR AND CELLULAR BIOCHEMISTRY, 2002, 230 (1-2) :139-147
[30]  
Peersen OB, 1997, PROTEIN SCI, V6, P794